Information on EC 6.2.1.27 - 4-hydroxybenzoate-CoA ligase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
6.2.1.27
-
RECOMMENDED NAME
GeneOntology No.
4-hydroxybenzoate-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 4-hydroxybenzoate + CoA = AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
forming of carbon sulfur bonds
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-coumarate degradation (anaerobic)
-
-
Aminobenzoate degradation
-
-
Benzoate degradation
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
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-
phenol degradation
-
-
phenol degradation II (anaerobic)
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-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxybenzoate:CoA ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
119699-80-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain K172
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-
Manually annotated by BRENDA team
strain K172
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 4-aminobenzoate + CoA
AMP + 4-aminobenzoyl-CoA + diphosphate
show the reaction diagram
-
30% of the activity with 4-hydroxybenzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + 4-fluorobenzoyl-CoA + diphosphate
show the reaction diagram
-
3.8% of the activity with 4-hydroxybenzoate
-
-
?
ATP + 4-hydroxybenzoate + CoA
AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
ATP + phenylacetate + CoA
AMP + phenylacetyl-CoA + diphosphate
show the reaction diagram
-
4.5% of the activity with 4-hydroxybenzoate
-
-
?
CTP + 4-hydroxybenzoate + CoA
CMP + 4-hydroxybenzoyl-CoA + diphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 4-hydroxybenzoate + CoA
AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4-Dihydroxybenzoate
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strong
2-hydroxy-4-methylbenzoate
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strong
5,5'-dithiobis(2-nitrobenzoate)
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037 - 0.34
4-hydroxybenzoate
0.077
ATP
-
pH 7.8, 37C
0.125
coenzyme A
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pH 7.8, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24 - 30
4-hydroxybenzoate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
70
4-hydroxybenzoate
Thauera aromatica
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pH and temperature not specified in the publication
365
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0012
2,4-Dihydroxybenzoate
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pH 7.8, 37C
0.0014
2-hydroxy-4-methylbenzoate
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pH 7.8, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
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using benzoate as a substrate
0.0014
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using p-hydroxybenzoate as a substrate
0.012
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using p-cresol as a substrate
4.12
-
pH 7.8, 30C
4.8
pH and temperature not specified in the publication
additional information
-
no specific activity using acetate as a substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.2 - 8.5
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-
9
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or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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50% of activity maximum at pH 7.0 and 9.5, almost no activity at pH 6.0 and 10.0
7 - 9
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pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
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gel filtration
50000
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gel filtration
60000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive during initial stages of purification, can be stabilized by adding DTT and 10% glycerol
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648909
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose chromatography, Reactive Green cross-linked agarose column chromatography, and Superdex 200 HiLoad 16/60 gel filtration
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strain K172
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of the C-terminal His6-tagged protein in Escherichia coli BL21
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the gene product is expressed as a C-terminal His10-tagged protein in Escherichia coli BL21(DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
induction during anaerobic growth with by 3-hydroxybenzoate
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