Information on EC 6.2.1.25 - Benzoate-CoA ligase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
6.2.1.25
-
RECOMMENDED NAME
GeneOntology No.
Benzoate-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + benzoate + CoA = AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acid-thiol ligation
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Aminobenzoate degradation
-
anaerobic aromatic compound degradation (Thauera aromatica)
-
benzoate biosynthesis I (CoA-dependent, beta-oxidative)
-
Benzoate degradation
-
benzoate degradation II (aerobic and anaerobic)
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
salicortin biosynthesis
-
tetrahydroxyxanthone biosynthesis (from benzoate)
-
SYSTEMATIC NAME
IUBMB Comments
Benzoate:CoA ligase (AMP-forming)
Also acts on 2-, 3- and 4-fluorobenzoate, but only very slowly on the corresponding chlorobenzoates.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
BamY
Geobacter metallireducens strain GS-15
Q39TQ2
-
-
Bcl
Magnetospirillum sp. strainTS-6
Q25C16
-
-
benzoate CoA ligase
-
-
benzoate CoA ligase
A1EAJ3
-
benzoate CoA ligase
Xanthomonas albilineans LS155
A1EAJ3
-
-
Benzoate coenzyme A ligase
-
-
-
-
Benzoate-CoA ligase (AMP-forming)
-
-
-
-
Benzoyl coenzyme A synthetase
-
-
-
-
Benzoyl-CoA ligase
-
-
-
-
Benzoyl-CoA synthetase
-
-
-
-
BzdA enzyme
-
-
BzdA enzyme
-
-
-
Synthetase, benzoyl coenzyme A
-
-
-
-
XabE
A1EAJ3
gene name
XabE
Xanthomonas albilineans LS155
A1EAJ3
gene name
-
CAS REGISTRY NUMBER
COMMENTARY
95329-17-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Anaerobic bacterium
a strictly anaerobic, obligate synthrophic mixed culture
-
-
Manually annotated by BRENDA team
gram-negative, nitrate-reducing strain Asl-3
-
-
Manually annotated by BRENDA team
strain LB400
-
-
Manually annotated by BRENDA team
annual California plant
-
-
Manually annotated by BRENDA team
ATCC 53774, DSM 7210
UniProt
Manually annotated by BRENDA team
Geobacter metallireducens strain GS-15
ATCC 53774, DSM 7210
UniProt
Manually annotated by BRENDA team
strainTS-6
TrEMBL
Manually annotated by BRENDA team
Magnetospirillum sp. strainTS-6
strainTS-6
TrEMBL
Manually annotated by BRENDA team
an anaerobically induced benzoate-CoA ligase and an aerobically induced benzoate-CoA ligase with different properties; strain KB740
-
-
Manually annotated by BRENDA team
Pseudomonas-like strain S100
-
-
Manually annotated by BRENDA team
strain KB740
-
-
Manually annotated by BRENDA team
Pseudomonas sp. KB740
strain KB740
-
-
Manually annotated by BRENDA team
male Wistar LAT1
-
-
Manually annotated by BRENDA team
strain SB, in syntropic association with Methanospillum hungatei strain JF1
-
-
Manually annotated by BRENDA team
strain DSM 6984
-
-
Manually annotated by BRENDA team
diverse mutant strains, gene xabE
UniProt
Manually annotated by BRENDA team
Xanthomonas albilineans LS155
diverse mutant strains, gene xabE
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
A1EAJ3, -
regulatory and synthase genes downstream of xabE, overview
metabolism
-, Q39TQ2
BamY is involved in benzene metabolism, detailed pathway overview
metabolism
Geobacter metallireducens strain GS-15
-
BamY is involved in benzene metabolism, detailed pathway overview
-
metabolism
Xanthomonas albilineans LS155
-
regulatory and synthase genes downstream of xabE, overview
-
physiological function
A1EAJ3, -
XabE is similar to benzoate CoA ligases and is essential for albicidin production and pathogenicity
physiological function
Xanthomonas albilineans LS155
-
XabE is similar to benzoate CoA ligases and is essential for albicidin production and pathogenicity
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
60% of the activity relative to benzoate
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
28% of the activity relative to benzoate
-
-
-
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
50% of the activity relative to benzoate
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
3% of the activity with benzoate
-
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
35% of the activity with benzoate
-
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
12.7% activity compared to benzoate
-
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-, Q25C16
6.7% and 10% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
-
-
-
-
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
28% of the activity relative to benzoate
-
-
-
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
-
35% of the activity with benzoate
-
-
?
ATP + 2-aminobenzoate + CoA
AMP + diphosphate + 2-aminobenzoyl-CoA
show the reaction diagram
Magnetospirillum sp. strainTS-6
Q25C16
6.7% and 10% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
ATP + 2-chlorobenzoate + CoA
AMP + diphosphate + 2-chlorobenzoyl-CoA
show the reaction diagram
-
10% of the activity relative to benzoate
-
-
-
ATP + 2-chlorobenzoate + CoA
AMP + diphosphate + 2-chlorobenzoyl-CoA
show the reaction diagram
-
5% of the activity with benzoate
-
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
102% of the activity relative to benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
120% of the activity relative to benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
Anaerobic bacterium
-
93% of the activity relative to benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
as effective as benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
135% of the activity relative to benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
122% of the activity relative to benzoate
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
91% of the activity with benzoate
-
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
92% of the activity with benzoate
-
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
1.9% activity compared to benzoate
-
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-, Q25C16
61% and 57% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
-
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
as effective as benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
135% of the activity relative to benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
120% of the activity relative to benzoate
-
-
-
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
-
91% of the activity with benzoate
-
-
?
ATP + 2-fluorobenzoate + CoA
AMP + diphosphate + 2-fluorobenzoyl-CoA
show the reaction diagram
Magnetospirillum sp. strainTS-6
Q25C16
61% and 57% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
ATP + 2-hydroxybenzoate + CoA
AMP + diphosphate + 2-hydroxybenzoyl-CoA
show the reaction diagram
-
15% of the activity relative to benzoate
-
?
ATP + 2-methylbenzoate + CoA
AMP + diphosphate + 2-methylbenzoyl-CoA
show the reaction diagram
-
no activity with anaerobically induced enzyme, 15% of the activity relative to benzoate with the aerobically induced enzyme
-
-
-
ATP + 3-aminobenzoate + CoA
AMP + diphosphate + 3-aminobenzoyl-CoA
show the reaction diagram
-, Q25C16
3.7% activity in anaerobically grown cells, compared to the activity with benzoate
-
-
?
ATP + 3-chlorobenzoate + CoA
AMP + diphosphate + 3-chlorobenzoyl-CoA
show the reaction diagram
-
13% of the activity with benzoate
-
-
?
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
-
-
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
-
98% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
-
80% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
-
76% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
Anaerobic bacterium
-
56% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
Pseudomonas sp. KB740
-
98% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
Pseudomonas sp. KB740
-
80% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoylCoA
show the reaction diagram
Pseudomonas sp. KB740
-
76% of the activity relative to benzoate
-
-
-
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoyl-CoA
show the reaction diagram
-
89% of the activity relative to benzoate
-
?
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoyl-CoA
show the reaction diagram
-
23% of the activity with benzoate
-
-
?
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoyl-CoA
show the reaction diagram
-
92% of the activity with benzoate
-
-
?
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoyl-CoA
show the reaction diagram
-, Q25C16
16% activity in aerobically and anaerobically grown cells, compared to the activity with benzoate
-
-
?
ATP + 3-fluorobenzoate + CoA
AMP + diphosphate + 3-fluorobenzoyl-CoA
show the reaction diagram
-
2.0% activity compared to benzoate
-
-
?
ATP + 3-hydroxybenzoate + CoA
AMP + diphosphate + 3-hydroxybenzoyl-CoA
show the reaction diagram
-
-
-
?
ATP + 3-hydroxybenzoate + CoA
AMP + diphosphate + 3-hydroxybenzoyl-CoA
show the reaction diagram
-
10% of the activity relative to benzoate
-
?
ATP + 3-hydroxybenzoate + CoA
AMP + diphosphate + 3-hydroxybenzoyl-CoA
show the reaction diagram
-
8% of the activity with benzoate, no activity is observed when ATP is replaced by GTP
-
-
?
ATP + 3-hydroxybenzoate + CoA
AMP + diphosphate + 3-hydroxybenzoyl-CoA
show the reaction diagram
-
1.1% activity compared to benzoate
-
-
?
ATP + 4-aminobenzoate + CoA
AMP + diphosphate + 4-aminobenzoyl-CoA
show the reaction diagram
-, Q25C16
5.6% activity in anaerobically grown cells, compared to the activity with benzoate
-
-
?
ATP + 4-chlorobenzoate + CoA
AMP + diphosphate + 4-chlorobenzoyl-CoA
show the reaction diagram
-
3% of the activity with benzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
as effective as benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
79% of the activity relative to benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
145% of the activity relative to benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
Anaerobic bacterium
-
75% of the activity relative to benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
72% of the activity relative to benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
100% of the activity relative to benzoate
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
84% of the activity with benzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
98% of the activity with benzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
1.4% activity compared to benzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-, Q25C16
72% and 69% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
79% of the activity relative to benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
145% of the activity relative to benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
as effective as benzoate
-
-
-
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
-
84% of the activity with benzoate
-
-
?
ATP + 4-fluorobenzoate + CoA
AMP + diphosphate + 4-fluorobenzoyl-CoA
show the reaction diagram
Magnetospirillum sp. strainTS-6
Q25C16
72% and 69% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
ATP + 4-hydroxy-3-methoxybenzoate + CoA
AMP + diphosphate + 4-hydroxy-3-methoxybenzoyl-CoA
show the reaction diagram
-
15% of the activity relative to benzoate
-
?
ATP + 4-hydroxybenzoate + CoA
AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
-
-
-
?
ATP + 4-hydroxybenzoate + CoA
AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
-
25% of the activity relative to benzoate
-
?
ATP + 4-hydroxybenzoate + CoA
AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
-
16% of the activity with benzoate, no activity is observed when ATP is replaced by GTP
-
-
?
ATP + 4-hydroxybenzoate + CoA
AMP + diphosphate + 4-hydroxybenzoyl-CoA
show the reaction diagram
-
1.0% activity compared to benzoate
-
-
?
ATP + acetate + CoA
AMP + diphosphate + acetyl-CoA
show the reaction diagram
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
Anaerobic bacterium
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-, Q39TQ2
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
100% activity
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
the enzyme catalyzes the initial step of benzoate metabolism
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-, Q25C16
100% activity in aerobically and anaerobically grown cells
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
Geobacter metallireducens strain GS-15
Q39TQ2
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
Pseudomonas sp. KB740
-
-
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
Magnetospirillum sp. strainTS-6
Q25C16
100% activity in aerobically and anaerobically grown cells
-
-
?
ATP + benzoate + CoA
?
show the reaction diagram
-
enzyme is involved in anaerobic degradation of benzoate
-
-
-
ATP + benzoate + CoA
?
show the reaction diagram
-
activation of benzoate to benzoyl-CoA, a more polar and nondiffusable molecule, decreases the concentration of benzoate in the cell, creating a gradient that favored the uptake of benzoate from the environment
-
-
-
ATP + benzoate + CoA
?
show the reaction diagram
-
first step of anaerobic benzoate degradation
-
-
-
ATP + cyclohex-1-ene-1-carboxylate + CoA
AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA
show the reaction diagram
-
13% of the activity with benzoate
-
-
?
ATP + cyclohex-3-ene-1-carboxylate + CoA
AMP + diphosphate + cyclohex-1-ene-1-carboxyl-CoA
show the reaction diagram
-
40% of the activity with benzoate
-
-
?
ATP + cyclohexa-1,5-diene-1-carboxylate + CoA
AMP + diphosphate + cyclohexa-1,5-diene-1-carboxyl-CoA
show the reaction diagram
-
23% of the activity relative to benzoate
-
?
ATP + cyclohexa-1-ene-1-carboxylate + CoA
AMP + diphosphate + cyclohexa-1-ene-1-carboxyl-CoA
show the reaction diagram
-
8% of the activity relative to benzoate
-
?
ATP + cyclohexanecarboxylate + CoA
AMP + diphosphate + cyclohexanecarboxyl-CoA
show the reaction diagram
-
7% of the activity with benzoate
-
-
?
ATP + DELTA1-cyclohexenecarboxylate + CoA
AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + DELTA1-cyclohexenecarboxylate + CoA
AMP + diphosphate + DELTA1-cyclohexenecarboxyl-CoA
show the reaction diagram
-
13% of the activity relative to benzoate
-
-
-
ATP + DELTA3-cyclohexenecarboxylate + CoA
AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + DELTA3-cyclohexenecarboxylate + CoA
AMP + diphosphate + DELTA3-cyclohexenecarboxyl-CoA
show the reaction diagram
-
54% of the activity relative to benzoate
-
-
-
ATP + glutarate + CoA
AMP + diphosphate + glutaryl-CoA
show the reaction diagram
-
-
-
?
ATP + isonicotinate + CoA
AMP + diphosphate + isonicotinyl-CoA
show the reaction diagram
Anaerobic bacterium
-
29% of the activity relative to benzoate
-
-
-
ATP + isonicotinate + CoA
AMP + diphosphate + isonicotinyl-CoA
show the reaction diagram
-
95% of the activity with benzoate
-
-
?
ATP + nicotinate + CoA
AMP + diphosphate + nicotinyl-CoA
show the reaction diagram
-
12% of the activity relative to benzoate
-
-
-
ATP + nicotinate + CoA
AMP + diphosphate + nicotinyl-CoA
show the reaction diagram
Anaerobic bacterium
-
11% of the activity relative to benzoate
-
-
-
ATP + nicotinate + CoA
AMP + diphosphate + nicotinyl-CoA
show the reaction diagram
-
32% of the activity with benzoate
-
-
?
ATP + phenylacetate + CoA
AMP + diphosphate + phenylacetyl-CoA
show the reaction diagram
-
-
-
?
ATP + phenylacetate + CoA
AMP + diphosphate + phenylacetyl-CoA
show the reaction diagram
-
10% of the activity relative to benzoate
-
?
ATP + phenylpropionate + CoA
AMP + diphosphate + phenylpropionyl-CoA
show the reaction diagram
-
10% of the activity relative to benzoate
-
?
ATP + picolinate + CoA
AMP + diphosphate + picolinyl-CoA
show the reaction diagram
-
-
-
-
-
ATP + picolinate + CoA
AMP + diphosphate + picolinyl-CoA
show the reaction diagram
Anaerobic bacterium
-
46% of the activity relative to benzoate
-
-
-
ATP + picolinate + CoA
AMP + diphosphate + picolinyl-CoA
show the reaction diagram
-
45% of the activity relative to benzoate
-
-
-
ATP + picolinate + CoA
AMP + diphosphate + picolinyl-CoA
show the reaction diagram
-, Q25C16
3.4% and 2.7% activity in aerobically and anaerobically grown cells, respectively, compared to the activity with benzoate
-
-
?
additional information
?
-
-
not 3-hydroxybenzoate
-
-
-
additional information
?
-
-
no reaction with GTP
-
-
-
additional information
?
-
-
no reaction with cinnamic, coumaric, ferulic, caffeic, p-aminobenzoic, octanoic, or 2,5-dihydroxybenzoic acid
-
?
additional information
?
-
-
benzoate degradation in Azoarcus sp. strain CIB is subject to carbon catabolic repression by some organic acids, indicating the existence of a physiological control that connects the expression of the bzd genes to the metabolic status of the cell
-
-
-
additional information
?
-
-, Q25C16
little or no activity with 2-hydroxybenzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-chlorobenzoate, 3-chlorobenzoate, 4-chlorobenzoate, cyclohexane carboxylate, and nicotinate
-
-
-
additional information
?
-
-
no or less than 1% activity with 2-methylbenzoate, 4-methylbenzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-bromobenzoate, 3-bromobenzoate, 4-bromobenzoate, phenylacetate, acetate, succinate, propionate, butyrate, and hexanoate
-
-
-
additional information
?
-
Pseudomonas sp. KB740
-
not 3-hydroxybenzoate
-
-
-
additional information
?
-
-
benzoate degradation in Azoarcus sp. strain CIB is subject to carbon catabolic repression by some organic acids, indicating the existence of a physiological control that connects the expression of the bzd genes to the metabolic status of the cell
-
-
-
additional information
?
-
Magnetospirillum sp. strainTS-6
Q25C16
little or no activity with 2-hydroxybenzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, 2-chlorobenzoate, 3-chlorobenzoate, 4-chlorobenzoate, cyclohexane carboxylate, and nicotinate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-, Q39TQ2
-
-
-
?
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
-
the enzyme catalyzes the initial step of benzoate metabolism
-
-
?
ATP + benzoate + CoA
?
show the reaction diagram
-
enzyme is involved in anaerobic degradation of benzoate
-
-
-
ATP + benzoate + CoA
?
show the reaction diagram
-
activation of benzoate to benzoyl-CoA, a more polar and nondiffusable molecule, decreases the concentration of benzoate in the cell, creating a gradient that favored the uptake of benzoate from the environment
-
-
-
ATP + benzoate + CoA
?
show the reaction diagram
-
first step of anaerobic benzoate degradation
-
-
-
ATP + benzoate + CoA
AMP + diphosphate + benzoyl-CoA
show the reaction diagram
Geobacter metallireducens strain GS-15
Q39TQ2
-
-
-
?
additional information
?
-
-
benzoate degradation in Azoarcus sp. strain CIB is subject to carbon catabolic repression by some organic acids, indicating the existence of a physiological control that connects the expression of the bzd genes to the metabolic status of the cell
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
restores activity partially after treatment with EDTA
Mg2+
-
restores activity after treatment with EDTA
Mg2+
Anaerobic bacterium
-
optimal concentration: 5 mM MgCl2; required
Mg2+
-
required
Mn2+
-
restores activity after treatment with EDTA
Mn2+
-
can replace Mg2+
Mn2+
Anaerobic bacterium
-
can replace Mg2+; optimal concentration: 5 mM MnCl2, 85% of the efficiency compared to MgCl2
Mn2+
-
can replace Mg2+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,4,5-trichlorophenoxyacetic acid
-
concentration dependent inhibitor
2,4-Dichlorophenoxyacetic acid
-
concentration dependent inhibitor
2-Hydroxybenzoate
-
competitive
5,5'-dithiobis(2-nitrobenzoate)
-
-
benzoyl-CoA
-
product inhibition
CoCl2
Anaerobic bacterium
-
-
CuCl2
Anaerobic bacterium
-
CuCl2
EDTA
Anaerobic bacterium
-
-
FeCl2
Anaerobic bacterium
-
-
n-octylglucoside
-
weak
NiCl2
Anaerobic bacterium
-
-
p-chloromercuribenzoate
-
-
Tween 100
-
-
-
Zn2+
Anaerobic bacterium
-
ZnCl2
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.15
-
2-Aminobenzoate
-
37C
0.25
-
2-Aminobenzoate
-
in Tris-HCl at pH 8.0
0.25
-
2-Aminobenzoate
-
-
0.5
-
2-Aminobenzoate
-
-
0.04
-
2-Fluorobenzoate
-
4-fluorobenzoate
0.09
-
2-Fluorobenzoate
-
aerobically induced enzyme
0.095
-
ATP
-
pH 7.5, 22C
0.125
-
ATP
-
anaerobically induced enzyme
0.13
-
ATP
-
-
0.2
-
ATP
-
coenzyme A
0.265
-
ATP
-, Q25C16
apparent value, at pH 9.0
0.27
-
ATP
-
in Tris-HCl at pH 8.0
0.277
-
ATP
-
aerobically induced enzyme
0.44
-
ATP
-
37C
0.56
-
ATP
Anaerobic bacterium
-
-
0.0006
0.002
Benzoate
-
-
0.009
-
Benzoate
-
anaerobically induced enzyme
0.01
-
Benzoate
-
-
0.011
-
Benzoate
-
-
0.011
-
Benzoate
-
benzoate, , aerobically induced enzyme
0.016
-
Benzoate
-
37C
0.03
-
Benzoate
-, Q25C16
apparent value, at pH 9.0
0.03
-
Benzoate
Anaerobic bacterium
-
-
0.045
-
Benzoate
-
pH 7.5, 22C
0.12
-
Benzoate
-
in Tris-HCl at pH 8.0
0.089
-
CoA
-
anaerobically induced enzyme
0.092
-
CoA
-, Q25C16
apparent value, at pH 9.0
0.105
-
CoA
-
aerobically induced enzyme
0.13
-
CoA
-
pH 7.5, 22C
0.18
-
CoA
-
in Tris-HCl at pH 8.0
0.06
-
CoASH
Anaerobic bacterium
-
-
0.1
-
CoASH
-
2-fluorobenzoate; CoA
0.1
-
CoASH
-
2-fluorobenzoate, , anaerobically induced enzyme
0.18
-
CoASH
-
37C
0.09
0.12
reduced CoA
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
433
-
Benzoate
-
-
additional information
-
additional information
-
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.016
-
2-Hydroxybenzoate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.07
-
-
pH 7.5, 22C
0.081
-
-, Q25C16
soluble protein, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10mM KCl, 2.5mM MgCl2, 10mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2U of lactate dehydrogenase, and 0.1 mM substrate
13.4
-
-, Q25C16
after 166fold purification, for benzoyl-CoA synthesis, in 100 mM Tris-HCl (pH 7.8), 10 mM KCl, 2.5 mM MgCl2, 10 mM phospho(enol)-pyruvate, 0.5 mM ATP, 0.25 mM CoA, 0.2 mM NADH, 2 units myokinase, 2 units pyruvate kinase, 2 units of lactate dehydrogenase, and 0.1 mM substrate
16.5
-
-
37C
73
-
-
-
additional information
-
Anaerobic bacterium
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.2
8.4
-
-
8
-
Anaerobic bacterium
-
-
9
-
-, Q25C16
-
9
-
-
aerobically induced enzyme
9.3
-
-
-
9.3
-
-
anaerobically induced enzyme
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.4
9.2
-
-
7
8.9
-
7.0: 10% of maximal activity, 8.0: 75% of maximal activity, 8.4-8.9: maximal activity
7
9
Anaerobic bacterium
-
7: about 40% of maximal activity, 9: about 30% of maximal activity
7
9.5
-, Q25C16
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
Anaerobic bacterium
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
45
Anaerobic bacterium
-
22C: about 40% of maximal activity, 45C: about 20% of maximal activity
PDB
SCOP
CATH
ORGANISM
Burkholderia xenovorans (strain LB400)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
58000
-
-
gel filtration
58000
-
-
HPLC gel filtration
59000
-
-
gel filtration
60000
62000
-
HPLC gel filtration
60000
-
-
gel filtration
64500
-
-
Comassie blue-stained gel filtration
100000
-
-
gel filtration
120000
-
-, Q25C16
SDS-PAGE
120000
-
-
gel filtration, anaerobically induced enzyme
126000
-
-, Q25C16
gel filtration
130000
-
-
gel filtration, aerobically induced enzyme
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Anaerobic bacterium
-
7 or 8 * 58000, SDS-PAGE
dimer
-
2 * 54000, SDS-PAGE, anaerobically induced enzyme; 2 * 56000, SDS-PAGE, aerobically induced enzyme
dimer
Pseudomonas sp. KB740
-
2 * 54000, SDS-PAGE, anaerobically induced enzyme; 2 * 56000, SDS-PAGE, aerobically induced enzyme
-
homodimer
-, Q25C16
2 * 63000, gel filtration
homodimer
Magnetospirillum sp. strainTS-6
-
2 * 63000, gel filtration
-
monomer
-
1 * 64500, gel filtration
monomer
-
1 * 58000 gel filtration
monomer
-
1 * 60000, SDS-PAGE
monomer
-
1 * 60000, SDS-PAGE
monomer
-
1 * 60000-62000, denaturing electrophoresis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
acetylation of residue Lys512 inactivates BadA, deacetylation reactivates BadA. Acetyltransferase RpPat modifies BadA, and also modifies acetyl-CoA synthetase. At least two deacetylases reactivate BadAAc. One is SrtN, a sirtuintype NAD+-dependent deacetylase, the other deacetylase is lysine deacetylase LdaA, an acetate-forming protein deacetylase. LdaA reactivates HbaAc and AliAAc in vitro
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sitting-drop vapor diffusion method at 18 C in 23% PEG 3350 K and 0.2 M sodium formate
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purified enzyme is insensitive to oxygen
-
707
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, 1 M imidazole, 500 mM NaCl, 20 mM His/HCl, pH 7.9, 10% glycerol, several months and multiple freeze/thaw cycles
-
0C, 1 mg/ml purified enzyme, half-life is 8 days
-
in liquid N2, no greater losses of activity after 3-6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Anaerobic bacterium
-
Ni-NTA resin column chromatography
-
DEAE, anion-exchange, HAP, affinity on reactive red resin and anion-exchange on a Mono-Q chromatography
-
His-tagged recombinant enzyme
-
DEAE-Toyopearl column chromatography, Phenyl-Sepharose Fast Flow column chromatography, and Superose 12 gel filtration
-, Q25C16
at 4C, by a method that includes DEAE-sepharose, source 30Q and reactive green chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expressed in Escherichia coli strain BL21 Star (DE3)
-
gene bamY, DNA and amino acid sequence comparisons and genotyping
-, Q39TQ2
overexpression in Escherichia coli
-
expressed in Escherichia coli
-
expression in Streptomyces erythrea of a single additional gene encN from the enterocin pathway allows either production of phenyl-substituted polyketides without medium supplementation, or incorporation of a range of substituted phenyl starter units into such polyketides
-
expressed in Escherichia coli
-
gene xabE, DNA and amino acid sequence determination and analysis, genotyping, regulatory and synthase genes downstream of xabE, overview. Expression in Zea mays plants
A1EAJ3, -
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
A1EAJ3, -
insertional mutation in gene xabE, using suicide-vector pJP-XabE, leads to complete loss of albicidin production ability. The mutant fails to induce symptoms of leaf scald in maize plants, in contrast to wild-type Xanthomonas albilineans, which elicits characteristic white pencil line symptoms in inoculated maize leaves
additional information
Xanthomonas albilineans LS155
-
insertional mutation in gene xabE, using suicide-vector pJP-XabE, leads to complete loss of albicidin production ability. The mutant fails to induce symptoms of leaf scald in maize plants, in contrast to wild-type Xanthomonas albilineans, which elicits characteristic white pencil line symptoms in inoculated maize leaves
-
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
acetylation of residue Lys512 inactivates BadA, deacetylation reactivates BadA
-