Together with a hydrolase component (EC 220.127.116.11/EC 18.104.22.168) and a reductase component (EC 22.214.171.124), this enzyme forms a multienzyme fatty acid reductase complex that produces the long-chain aldehyde substrate of the bacterial luciferase enzyme (EC 126.96.36.199). The enzyme activates free long-chain fatty acids, generated by the action of the transferase component, forming a fatty acyl-AMP intermediate, followed by the transfer of the acyl group to an internal L-cysteine residue. It then transfers the acyl group to EC 188.8.131.52, long-chain acyl-protein thioester reductase.
two polypeptides of MW 34000 and MW 50000 being involved in the acyl-protein synthetase activity are identified. Acylation of the 50000 MW polypeptide alone occurs at a low rate. The rate and level of acylation are greatly stimulated by the addition of either the 34000 MW polypeptide or by the 58000 MW acyl-CoA reductase