Information on EC 6.2.1.15 - arachidonate-CoA ligase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
6.2.1.15
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RECOMMENDED NAME
GeneOntology No.
arachidonate-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + arachidonate + CoA = AMP + diphosphate + arachidonoyl-CoA
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
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SYSTEMATIC NAME
IUBMB Comments
arachidonate:CoA ligase (AMP-forming)
Not identical with EC 6.2.1.3 long-chain-fatty-acid---CoA ligase. Icosa-8,11,14-trienoate, but not the other long-chain fatty acids, can act in place of arachidonate.
CAS REGISTRY NUMBER
COMMENTARY hide
82047-87-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Wistar strain
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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The alternative pathway, in which the rate-limiting enzyme is ACSL4, provides arachidonoyl-CoA to a mitochondrial acyl-CoA thioesterase that releases AA in mitochondria and directs this fatty acid to the lipooxygenase for its subsequent conversion to lipooxygenase metabolites
physiological function
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the acyl-CoA synthetase, ACSL4, belongs to a five member family of enzymes that esterify mainly arachidonic acid into acyl-CoA. Role of ACSL4 and acyl-CoA thioesterase, ACOT2, in the production of lipooxygenase metabolites in breast cancer cells, regulation, overview. ACSL4 is the rate-limiting enzyme for intramitochondrial arachidonic acid generation and export, for the conversion to lipooxygenase metabolites
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5,8,11,14,17-eicosapentaenoic acid + CoA
ATP + diphosphate + 5,8,11,14,17-eicosapentaenoyl-CoA
show the reaction diagram
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ATP + 5,8,11-eicosatrienoate + CoA
AMP + diphosphate + 5,8,11-eicosatrienoyl-CoA
show the reaction diagram
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ATP + 8,11,14-eicosatrienoate + CoA
AMP + diphosphate + 8,11,14-eicosatrienoyl-CoA
show the reaction diagram
ATP + arachidonate + CoA
?
show the reaction diagram
ATP + arachidonate + CoA
AMP + diphosphate + arachidonoyl-CoA
show the reaction diagram
ATP + docosahexaenoate + CoA
ATP + diphosphate + docosahexaenoyl-CoA
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + arachidonate + CoA
?
show the reaction diagram
ATP + arachidonate + CoA
AMP + diphosphate + arachidonoyl-CoA
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,8,11-eicosatrienoate
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7,10,13,16-Docosatetraenoate
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8,11,14-Eicosatrienoate
arachidonate
docosahexaenoate
eicosapentaenoate
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slight
fatty acids
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overview
H2O2
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oleate
palmitate
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Triacsin C
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.041
5,8,11-eicosatrienoate
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0.0058 - 0.0793
arachidonate
0.029 - 2.39
ATP
0.005 - 0.15
CoA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
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7: about 50% of maximal activity, 9.5: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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coronary microvessel, cell culture
Manually annotated by BRENDA team
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a breast cancer cell model, comprising different cell lines, expresses ACSL4 in all cell lines, high expression level
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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7 min, 25% loss of activity
additional information
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heat inactivation does not distinguish between arachidonoyl-CoA synthetase and a palmitoyl-CoA synthetase
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, stable for up to 6 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
functional overexpression of ACSL4 variant 1 in smooth muscle cells using a retroviral vector, semiquantitative and quantitative real-time PCR expression analysis
overexpression of ACSL4 in the MCF-7 Tet-off/ACSL4 cell line affects cell proliferation
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information