Information on EC 6.2.1.14 - 6-carboxyhexanoate-CoA ligase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
6.2.1.14
-
RECOMMENDED NAME
GeneOntology No.
6-carboxyhexanoate-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 6-carboxyhexanoate + CoA = AMP + diphosphate + 6-carboxyhexanoyl-CoA
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
8-amino-7-oxononanoate biosynthesis III
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-
Biotin metabolism
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Metabolic pathways
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-
SYSTEMATIC NAME
IUBMB Comments
6-carboxyhexanoate:CoA ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
55467-50-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain NIHB 12
-
-
Manually annotated by BRENDA team
strain NIHB 12
-
-
Manually annotated by BRENDA team
strain IAM 1193, weak
-
-
Manually annotated by BRENDA team
strain IAM 1193, weak
-
-
Manually annotated by BRENDA team
strain IFO 3140
-
-
Manually annotated by BRENDA team
strain IFO 3140
-
-
Manually annotated by BRENDA team
strain AKU 0022, strain IFO 3317, strain IFO 12010
-
-
Manually annotated by BRENDA team
strain AKU 0007
-
-
Manually annotated by BRENDA team
strain AKU 0007
-
-
Manually annotated by BRENDA team
strain IAM 1257
-
-
Manually annotated by BRENDA team
strain IFO 3764
-
-
Manually annotated by BRENDA team
Lavandula vera
biotin-overproducing cell line
-
-
Manually annotated by BRENDA team
strain IFO 3525
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain AKU 0821
-
-
Manually annotated by BRENDA team
strain AKU 0821
-
-
Manually annotated by BRENDA team
strain 35
SwissProt
Manually annotated by BRENDA team
strain 35
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + heptanedioate + CoA
AMP + phosphate + heptanedioyl-CoA
show the reaction diagram
ATP + heptanedioate + CoA
?
show the reaction diagram
ATP + heptanedioate + CoA
AMP + diphosphate + heptanedioyl-CoA
show the reaction diagram
ATP + hexanedioate + CoA
AMP + diphosphate + hexanedioyl-CoA
show the reaction diagram
ATP + nonanedioate + CoA
AMP + diphosphate + nonanedioyl-CoA
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + heptanedioate + CoA
?
show the reaction diagram
ATP + heptanedioate + CoA
AMP + diphosphate + heptanedioyl-CoA
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
32% of the activation relative to Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2,2'-dipyridyl
-
-
iodoacetic acid
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-
p-chloromercuribenzoate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.72 - 1.5
ATP
0.033 - 0.55
CoA
0.145 - 0.49
heptanedioate
0.17
MgATP2-
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-
additional information
additional information
Michaelis-Menten kinetic
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
Lysinibacillus sphaericus
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-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
77.3
freshly prepared enzyme, pH 8.0, at 30C
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9.5
-
8: about 55% of maximal activity, 9.5: about 65% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 52
-
15C: about 50% of maximal activity, 52C: about 15% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
pH-gradient 6.0-4.0, chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Aquifex aeolicus (strain VF5)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
2 * 28000, SDS-PAGE
53000
-
x * 53000, SDS-PAGE
60000
-
gel filtration
74643
4 * 74643, calculation from sequence of cDNA
298600
calculated from sequence of cDNA
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 53000, SDS-PAGE
tetramer
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anion exchange, gel filtration, chromatofocusing
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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