Information on EC 6.2.1.11 - biotin-CoA ligase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
6.2.1.11
-
RECOMMENDED NAME
GeneOntology No.
biotin-CoA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + biotin + CoA = AMP + diphosphate + biotinyl-CoA
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid-thiol ligation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biotin metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
biotin:CoA ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37318-60-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
No. 166
-
-
Manually annotated by BRENDA team
Soil bacterium
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 5-(2-thienyl)-n-valerate + CoA
AMP + diphosphate + 5-(2-thienyl)-n-valeryl-CoA
show the reaction diagram
ATP + actithiazic acid + CoA
AMP + diphosphate + actithiazyl-CoA
show the reaction diagram
ATP + alpha-dehydrobiotin + CoA
AMP + diphosphate + alpha-dehydrobiotinyl-CoA
show the reaction diagram
-
27% of the activity relative to biotin
-
-
-
ATP + biotin + CoA
?
show the reaction diagram
-
first enzyme involved in biotin degradation
-
-
-
ATP + biotin + CoA
AMP + diphosphate + biotinyl-CoA
show the reaction diagram
ATP + dethiobiotin + CoA
AMP + diphosphate + dethiobiotinyl-CoA
show the reaction diagram
ATP + n-caprylate + CoA
AMP + diphosphate + n-capryloyl-CoA
show the reaction diagram
-
19% of the activity relative to biotin
-
-
-
ATP + n-decanoate + CoA
AMP + diphosphate + n-decanoyl-CoA
show the reaction diagram
-
14% of the activity relative to biotin
-
-
-
ATP + n-laurate + CoA
AMP + diphosphate + n-lauroyl-CoA
show the reaction diagram
-
11% of the activity relative to biotin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + biotin + CoA
?
show the reaction diagram
-
first enzyme involved in biotin degradation
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,2'-dipyridyl
-
-
iodoacetic acid
-
-
p-chloromercuribenzoate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.32
(+)-biotin
-
-
0.3
(+/-)-dethiobiotin
-
-
1.3
5-(2-thienyl)-n-valerate
-
-
2.8
ATP
-
-
0.15
CoA
-
-
0.43
DL-actithiazic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.89
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000 - 57000
-
-
57000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
-
25°C, 1 h, stable
589, 592
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 35
-
20 min, stable
25
-
pH 6.5-8.5, 1 h, stable
35
-
20 min, pH 7.4, stable
40
-
20 min, pH 7.4, 54% loss of activity
45
-
20 min, pH 7.4, 85% loss of activity
50
-
20 min, pH 7.4, 93% loss of activity
60
-
20 min, pH 7.4, 96% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
bicarbonate stabilizes during DEAE-cellulose chromatography
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, enzyme loses almost all of its activity on storage for 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
enzymatic assay for biotin
synthesis
-
production of alpha-dehydrobiotin, an antibiotic, from biotinyl-CoA with biotinyl-CoA synthetase and acyl-CoA oxidase