Information on EC 6.1.2.2 - nebramycin 5' synthase

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The expected taxonomic range for this enzyme is: Streptoalloteichus tenebrarius

EC NUMBER
COMMENTARY hide
6.1.2.2
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RECOMMENDED NAME
GeneOntology No.
nebramycin 5' synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
carbamoyl phosphate + ATP + H2O = diphosphate + O-carbamoyladenylate + phosphate
show the reaction diagram
(1a); (2a)
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kanamycin A + carbamoyl phosphate + ATP + H2O = 6''-O-carbamoylkanamycin A + AMP + diphosphate + phosphate
show the reaction diagram
(2) overall reaction
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O-carbamoyladenylate + kanamycin A = AMP + 6''-O-carbamoylkanamycin A
show the reaction diagram
catalytic reaction mechanism, detailed overview
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O-carbamoyladenylate + tobramycin = AMP + nebramycin 5'
show the reaction diagram
(1b)
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tobramycin + carbamoyl phosphate + ATP + H2O = nebramycin 5' + AMP + diphosphate + phosphate
show the reaction diagram
(1) overall reaction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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Neomycin, kanamycin and gentamicin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
tobramycin:carbamoyl phosphate ligase (AMP,phosphate-forming)
Requires Fe(III). The enzyme from the bacterium Streptoalloteichus tenebrarius catalyses the activation of carbamoyl phosphate to O-carbamoyladenylate and the subsequent carbamoylation of kanamycin and tobramycin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tobramycin + carbamoyl phosphate + ATP + H2O
nebramycin 5' + AMP + diphosphate + phosphate
show the reaction diagram
-
overall reaction
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tobramycin + carbamoyl phosphate + ATP + H2O
nebramycin 5' + AMP + diphosphate + phosphate
show the reaction diagram
-
overall reaction
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
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contains iron
Mg2+
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contains Mg2+ or Mn2+
Mn2+
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contains Mg2+ or Mn2+
PDB
SCOP
CATH
ORGANISM
UNIPROT
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)
Streptoalloteichus tenebrarius (strain ATCC 17920 / DSM 40477 / NCIB 11028)