Any feedback?
Information on EC 6.1.1.22 - asparagine-tRNA ligase and Organism(s) Brugia malayi and UniProt Accession P10723
for references in articles please use BRENDA:EC6.1.1.22Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
6.1.1.22 asparagine-tRNA ligaseSpecify your search results
Word Map
- 6.1.1.22
- aminoacyl-trna
- asnrs
- aspartyl-trna
- malayi
- brugia
- aminoacylation
-
amidotransferase
-
asparaginylation
- asp-trnaasn
- glutaminyl-trna
- gatcabs
- filariasis
-
transamidation
-
nondiscriminating
- glu-trnagln
-
mischarged
-
misacylated
-
asn-trna
- nd-asprs
-
ammonia-dependent
-
anti-ks
- lysyl-trna
- trnaasp
-
histidyl-trna
- medicine
- drug development
The taxonomic range for the selected organisms is: Brugia malayi
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
asparaginyl-trna synthetase, nars2, asparagine synthetase a, as-ar, asparagyl-transfer rna synthetase, asparaginyl trna synthetase, asparagine-trna ligase, asparaginyl-transfer rna synthetase, asparagine trna synthetase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AsnRS
Asparagine translase
Asparagine--tRNA ligase
Asparaginyl transfer ribonucleic acid synthetase
Asparaginyl transfer RNA synthetase
Asparaginyl-transfer ribonucleate synthetase
Asparaginyl-tRNA synthetase
Asparagyl-transfer RNA synthetase
Potentially protective 63 kDa antigen
Synthetase, asparaginyl-transfer ribonucleate
AsnRS
-
-
-
-
Asparagine translase
-
-
-
-
Asparagine--tRNA ligase
-
-
-
-
Asparaginyl transfer ribonucleic acid synthetase
-
-
-
-
Asparaginyl transfer RNA synthetase
-
-
-
-
Asparaginyl-transfer ribonucleate synthetase
-
-
-
-
Asparaginyl-tRNA synthetase
-
-
-
-
Asparaginyl-tRNA synthetase
-
-
Asparagyl-transfer RNA synthetase
-
-
-
-
Potentially protective 63 kDa antigen
-
-
-
-
Synthetase, asparaginyl-transfer ribonucleate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
esterification
Aminoacylation
esterification
-
-
-
-
Aminoacylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
L-asparagine:tRNAAsn ligase (AMP-forming)
-
CAS REGISTRY NUMBER
COMMENTARY
37211-76-0
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
-
-
?
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
-
-
?
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
-
-
-
?
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
with the aminoacylation step E + AA + ATP = E(AA-AMP) + diphosphate, and the amidation step E(AA-AMP) + tRNA = E+AA-tRNA + AMP
-
-
r
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
AsnRS structure analysis shows binding of Mg2+-ATP in one active site and L-Asp-beta-NOH adenylate and Mg2+-diphosphate in the other active site. Active-site ordering and rearrangement during activation, overview. The AsnRSBm N-terminal extension binds tRNA, structure, overview
-
-
?
additional information
?
-
-
the high level of asparaginyl-tRNA synthetase expression reflects unusual metabolic demands associated with larval maturation
-
-
?
additional information
?
-
-
the parasite Brugia malayi enzyme, in analogy to the human enzyme, induces human leukocyte chemotaxis and activates G-protein-coupled receptors CXCR1 and CXCR2, but not CXCR3, filarial asparaginyl-tRNA synthetase, AsnRS, is known to be an immunodominant antigen that induces strong human immunoglobulin G3 responses and contributes to the development of chronic inflammatory disease such as lymphatic filariasis, overview
-
-
?
additional information
?
-
-
active site and ligand binding structure and mechanism, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
-
-
?
ATP + L-asparagine + tRNAAsn
AMP + diphosphate + L-asparaginyl-tRNAAsn
-
-
-
-
?
additional information
?
-
-
the high level of asparaginyl-tRNA synthetase expression reflects unusual metabolic demands associated with larval maturation
-
-
?
additional information
?
-
-
the parasite Brugia malayi enzyme, in analogy to the human enzyme, induces human leukocyte chemotaxis and activates G-protein-coupled receptors CXCR1 and CXCR2, but not CXCR3, filarial asparaginyl-tRNA synthetase, AsnRS, is known to be an immunodominant antigen that induces strong human immunoglobulin G3 responses and contributes to the development of chronic inflammatory disease such as lymphatic filariasis, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
asparaginyl sulfamoyl adenylate
-
i.e. ASNAMS, a non-hydrolyzable analogue of asparaginyl adenylate
NSC12156
-
i.e. N1,N3-bis(4-amino-2-methyl-6-quinolinyl)-2,2-dimethylmalonamide, 47% inhibition at 0.2 mM, docking mode and binding structure, overview
NSC35467
-
i.e. 2-(3-methyl-1 lambda5-pyridin-1-yl)-1-(2-phenanthryl)ethanone, 53% inhibition at 0.2 mM
NSC363624
-
i.e. 4-(3-(4-amino-6-isopropenyl-1,3,5-triazin-2-yl)phenyl)-6-isopropenyl-1,3,5-triazin-2-ylamine, 50% inhibition at 0.025 mM
rishirilide B
-
CSD code CUQZUJ, isolated from Streptomyces rishiriensis, has antithrombotic activity through selective alpha2-macroglobulin inhibition, leading to the activation of plasmin
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the enzyme is a highly expressed excretory-secretory molecule which activates interleukin 8 (IL-8) receptors via extracellular domains that are different from those used by IL-8
physiological function
-
the enzyme induces endothelial cell proliferation, promotes chemotaxis and induces ring formation and tube formation by EA.hy926 cells. The enzyme induces vasodilation in chick embryo chorioallantoic membranes
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SYNC_BRUMA
548
0
62339
Swiss-Prot
other Location (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
-
structure analysis using crystal structure, modeling the conformational flexibility of Brugia AsnRS, overview
additional information
additional information
-
X-ray crystallography and NMR structure determination and analysis of full-length and catalytically active N-terminally truncated enzyme, DELTAAsnRSBm monomer, comprising residues 112-548, overview
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
identification of peptidyl regions that are surface-accessible and available for antibody binding
purified apo-AsnRS, AsnRS in complex with AsnAMS, and in complex with Mg2+, ATP, and l-Asp-beta-NOH, X-ray diffraction structure determination and analysis at 1.9-2.4 A resolution
-
purified dimeric enzyme with bound Mg2+ and a non-hydrolyzable analogue of asparaginyl adenylate, ASNAMS, X-ray diffraction structure determination and analysis at 1.9 A resolution, modeling
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
treatment of HEK-293T cells expressing CXCR2 with the filarial AsnRS, recombinantly expressed in Escherichia coli, parasite AsnRS is chemotactic for human neutrophils and eosinophils, blocks CXCL1-induced calcium flux, and induces mitogen-activated protein kinase, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, multicopy gene, present in 5 gene clusters, the most abundant of all aminoacyl-tRNA synthetases in Brugia malayi
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
filariasis caused by the nematode, Brugia malayi, is an important worldwide tropical disease in which parasites disappear from the bloodstream during daylight hours, thus hampering standard microscopic diagnostic methods
drug development
-
Brugia malayi asparaginyl-tRNA synthetase, a target for anti-parasitic drug design
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kron, M.; Marquard, K.; Hrtlein, M.; Price, S.; Leberman, R.
An immunodominant antigen of Brugia malayi is an asparaginyl-tRNA synthetase
FEBS Lett.
374
122-124
1995
Brugia malayi
Kron, M.; Petridis, M.; Milev, Y.; Leykam, J.; Hartlein, M.
Expression, localization and alternative function of cytoplasmic asparaginyl-tRNA synthetase in Brugia malayi
Mol. Biochem. Parasitol.
129
33-39
2003
Brugia malayi
Sukuru, S.C.; Crepin, T.; Milev, Y.; Marsh, L.C.; Hill, J.B.; Anderson, R.J.; Morris, J.C.; Rohatgi, A.; O'Mahony, G.; Groetli, M.; Danel, F.; Page, M.G.; Haertlein, M.; Cusack, S.; Kron, M.A.; Kuhn, L.A.
Discovering new classes of Brugia malayi asparaginyl-tRNA synthetase inhibitors and relating specificity to conformational change
J. Comput. Aided Mol. Des.
20
159-178
2006
Brugia malayi, Homo sapiens
Ramirez, B.L.; Howard, O.M.; Dong, H.F.; Edamatsu, T.; Gao, P.; Hartlein, M.; Kron, M.
Brugia malayi asparaginyl-transfer RNA synthetase induces chemotaxis of human leukocytes and activates G-protein-coupled receptors CXCR1 and CXCR2
J. Infect. Dis.
193
1164-1171
2006
Brugia malayi, Homo sapiens
Kron, M.A.; Cichanowicz, S.; Hendrick, A.; Liu, A.; Leykam, J.; Kuhn, L.A.
Using structural analysis to generate parasite-selective monoclonal antibodies
Protein Sci.
17
983-989
2008
Brugia malayi (P10723), Brugia malayi
Crepin, T.; Peterson, F.; Haertlein, M.; Jensen, D.; Wang, C.; Cusack, S.; Kron, M.
A hybrid structural model of the complete Brugia malayi cytoplasmic asparaginyl-tRNA synthetase
J. Mol. Biol.
405
1056-1069
2011
Brugia malayi
Yu, Z.; Vodanovic-Jankovic, S.; Ledeboer, N.; Huang, S.X.; Rajski, S.R.; Kron, M.; Shen, B.
Tirandamycins from Streptomyces sp. 17944 inhibiting the parasite Brugia malayi asparagine tRNA synthetase
Org. Lett.
13
2034-2037
2011
Brugia malayi
Kron, M.A.; Wang, C.; Vodanovic-Jankovic, S.; Howard, O.M.; Kuhn, L.A.
Interleukin-8-like activity in a filarial asparaginyl-tRNA synthetase
Mol. Biochem. Parasitol.
185
66-69
2012
Brugia malayi
Rateb, M.; Yang, D.; Vodanovic-Jankovic, S.; Yu, Z.; Kron, M.; Shen, B.
Adipostatins A-D from Streptomyces sp. 4875 inhibiting Brugia malayi asparaginyl-tRNA synthetase and killing adult Brugia malayi parasites
J. Antibiot.
68
540-542
2015
Brugia malayi
-
EXTERNAL LINKS (specific for EC-Number 6.1.1.22)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
