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Information on EC 6.1.1.2 - tryptophan-tRNA ligase and Organism(s) Archaeoglobus fulgidus and UniProt Accession O28579

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Archaeoglobus fulgidus
UNIPROT: O28579 not found.
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The taxonomic range for the selected organisms is: Archaeoglobus fulgidus
The enzyme appears in selected viruses and cellular organisms
Synonyms
tryptophanyl-trna synthetase, trprs, wars2, mini-trprs, t2-trprs, tryptophanyl trna synthetase, htrprs, trprs1, trprs ii, trp-rs, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(Mt)TrpRS
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-
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hWRS
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-
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IFP53
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-
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Synthetase, tryptophanyl-transfer ribonucleate
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TrpRS
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Tryptophan translase
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Tryptophan--tRNA ligase
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Tryptophanyl ribonucleic synthetase
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Tryptophanyl-transfer ribonucleate synthetase
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Tryptophanyl-transfer ribonucleic acid synthetase
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Tryptophanyl-transfer ribonucleic synthetase
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Tryptophanyl-transfer RNA synthetase
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Tryptophanyl-tRNA synthase
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Tryptophanyl-tRNA synthetase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
esterification
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Aminoacylation
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-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
L-tryptophan:tRNATrp ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-44-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73, wild-type)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73C)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73G)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73G)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73U)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73, wild-type)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73C)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73G)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73G)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73U)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73, wild-type)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73A)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73A)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73C)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73U)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00006
Archeoglobus fulgidus tRNATrp(A73, wild-type)
pH 7.0, 65°C
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0.00005
Archeoglobus fulgidus tRNATrp(A73C)
pH 7.0, 65°C
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0.00006
Archeoglobus fulgidus tRNATrp(A73G)
pH 7.0, 65°C
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0.00005
Archeoglobus fulgidus tRNATrp(A73U)
pH 7.0, 65°C
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0.00009
Bacillus subtilis tRNATrp(A73, wild-type)
pH 7.0, 65°C
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0.00012
Bacillus subtilis tRNATrp(A73C)
pH 7.0, 65°C
-
0.0001
Bacillus subtilis tRNATrp(A73G)
pH 7.0, 65°C
-
0.00012
Bacillus subtilis tRNATrp(A73U)
pH 7.0, 65°C
-
0.00018
bovine tRNATrp(G73, wild-type)
pH 7.0, 65°C
-
0.00021
bovine tRNATrp(G73A)
pH 7.0, 65°C
-
0.00022
bovine tRNATrp(G73C)
pH 7.0, 65°C
-
0.00017
bovine tRNATrp(G73U)
pH 7.0, 65°C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.53
Archeoglobus fulgidus tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.11
Archeoglobus fulgidus tRNATrp(A73C)
pH 7.0, 65°C
-
0.07
Archeoglobus fulgidus tRNATrp(A73G)
pH 7.0, 65°C
-
0.09
Archeoglobus fulgidus tRNATrp(A73U)
pH 7.0, 65°C
-
0.14
Bacillus subtilis tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.06
Bacillus subtilis tRNATrp(A73C)
pH 7.0, 65°C
-
0.04
Bacillus subtilis tRNATrp(A73G)
pH 7.0, 65°C
-
0.05
Bacillus subtilis tRNATrp(A73U)
pH 7.0, 65°C
-
0.03
bovine tRNATrp(G73, wild-type)
pH 7.0, 65°C
-
0.08
bovine tRNATrp(G73A)
pH 7.0, 65°C
-
0.06
bovine tRNATrp(G73C)
pH 7.0, 65°C
-
0.04
bovine tRNATrp(G73U)
pH 7.0, 65°C
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Guo, Q.; Gong, Q.; Tong, K.L.; Vestergaard, B.; Costa, A.; Desgres, J.; Wong, M.; Grosjean, H.; Zhu, G.; Wong, J.T.; Xue, H.
Recognition by tryptophanyl-tRNA synthetases of discriminator base on tRNATrp from three biological domains
J. Biol. Chem.
277
14343-14349
2002
Archaeoglobus fulgidus (O28579), Archaeoglobus fulgidus
Manually annotated by BRENDA team