Information on EC 5.5.1.5 - Carboxy-cis,cis-muconate cyclase

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The expected taxonomic range for this enzyme is: Dikarya

EC NUMBER
COMMENTARY hide
5.5.1.5
-
RECOMMENDED NAME
GeneOntology No.
Carboxy-cis,cis-muconate cyclase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 3-carboxy-cis,cis-muconate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination/addition
-
-
intramolecular
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
3-carboxy-2,5-dihydro-5-oxofuran-2-acetate lyase (decyclizing)
-
CAS REGISTRY NUMBER
COMMENTARY hide
37318-55-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Neosartorya fischeri
var. glaber
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
deletion mutant cannot catabolize phenolic compounds known to be degraded via the beta-ketoadipate pathway. Mutant is impaired in root invasion and is nonpathogenic, even though it shows normal superficial root colonization
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
?
show the reaction diagram
3-Carboxy-cis,cis-muconate
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
show the reaction diagram
cis,cis-Muconate
5-Oxo-2,5-dihydrofuran-2-acetate
show the reaction diagram
-
-
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-Carboxy-5-oxo-2,5-dihydrofuran-2-acetate
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
contains 0.06 M to 0.3 M of Ca2+ per mol of subunit
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Chloro-4-methylmuconolactone
-
non-competitive
3-carboxy-cis,cis-muconate
-
substrate inhibition at concentration exceeding 0.070 mM
3-chloromuconate
-
competitive
3-Chloromuconolactone
-
non-competitive
3-methylmuconate
-
competitive
adipate
-
-
AsO43-
-
-
BO33-
-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
cis-aconitate
-
-
citraconate
-
-
citrate
-
-
Ethoxyformic anhydride
-
irreversible, presence of the competitive inhibitors cis-aconitate, trans-aconitate and cis,cis-muconate conferrs some protection
fumarate
-
-
glutaconate
-
-
Glutarate
-
-
Itaconate
-
-
malate
-
-
Maleate
-
-
malonate
-
-
mesaconate
-
-
Na2H2PO2
-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
-
oxalate
-
-
phosphate
-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
pimelate
-
-
S2O32-
-
negligible effect near the pH optimum but inhibits to a greater extent on the acid side of the optimum
succinate
-
-
Tartrate
-
-
trans,trans-muconate
-
-
trans-aconitate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0103 - 0.057
3-carboxy-cis,cis-muconate
26.2
cis,cis-muconate
-
recombinant enzyme expressed in E. coli
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
360 - 382
3-carboxy-cis,cis-muconate
1.1
cis,cis-muconate
Neurospora crassa
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
600
-
recombinant enzyme expressed in Escherichia coli
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6
-
-
7
-
broad around
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
158000
-
gel filtration
191000
-
high-speed equilibrium sedimentation
200000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
-
8 * 24000, equilibrium sedimentation in presence of 5 M guanidine hydrochloride and 2-mercaptoethanol, SDS-PAGE
tetramer
-
4 * 41000, SDS-PAGE; 4 * 41200, calculation from nucleotide sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
hexose composition of 9.54% which can be equivalent to 14.2 residues per polypeptide chain of MW 24000. An N-acetylglucosamine concentration of 2.8% is determined which can be equivalent to 3.9 residues per polypeptide chain
no glycoprotein
-
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
rose-bengal-sensitized photooxidation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for over 9 months in the intact mycelium
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-