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Information on EC 5.5.1.4 - inositol-3-phosphate synthase and Organism(s) Arabidopsis thaliana and UniProt Accession P42801
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5.5.1.4 inositol-3-phosphate synthaseIUBMB Comments
Requires NAD+, which dehydrogenates the -CHOH- group to -CO- at C-5 of the glucose 6-phosphate, making C-6 into an active methylene, able to condense with the -CHO at C-1. Finally, the enzyme-bound NADH reconverts C-5 into the -CHOH- form.
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Word Map
- 5.5.1.4
-
nucleation
-
neuroblast
-
phytic
-
ice-nucleating
-
monophosphatase
-
cloud
-
halophytic
-
inositol-containing
-
cyclitols
- phytate
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supercooled
- galactinol
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hexakisphosphate
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radiative
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2-kinase
- impase
- medicine
- pharmacology
- biotechnology
- synthesis
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
inps, myo-inositol-1-phosphate synthase, mips1, inositol-1-phosphate synthase, mip synthase, pip synthase, myo-inositol 1-phosphate synthase, inositol-3-phosphate synthase, l-myo-inositol-1-phosphate synthase, sll1981, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1L-myo-Inositol-1-phosphate synthase
-
-
-
-
D-Glucose 6-phosphate cycloaldolase
-
-
-
-
D-Glucose 6-phosphate-1L-myoinositol 1-phosphate cyclase
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-
-
-
D-Glucose 6-phosphate-1L-myoinositol 1-phosphate cycloaldolase
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-
-
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D-Glucose 6-phosphate-L-myo-inositol 1-phosphate cyclase
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-
-
-
D-Glucose-6-phosphate,L-myo-inositol-1-phosphate cycloaldolase
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-
-
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Glucocycloaldolase
-
-
-
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Glucose 6-phosphate cyclase
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-
-
-
Glucose-6-phosphate inositol monophosphate cycloaldolase
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-
-
-
Inositol 1-phosphate synthase
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-
-
-
Inositol 1-phosphate synthetase
-
-
-
-
INPS1
-
-
-
-
IPS
-
-
-
-
L-myo-Inositol 1-phosphate synthetase
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-
-
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L-myo-Inositol-1-phosphate synthase
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-
-
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MI-1-P synthase
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-
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MIP synthase
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-
-
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MIPS
myo-Inositol-1-P synthase
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-
-
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Myo-inositol-1-phosphate synthase
Synthase, myo-inositol 1-phosphate
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-
-
-
MIPS
-
-
-
-
Myo-inositol-1-phosphate synthase
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate
no upregulation of transcription by salinity stress
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cyclization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
1D-myo-inositol-3-phosphate lyase (isomerizing)
Requires NAD+, which dehydrogenates the -CHOH- group to -CO- at C-5 of the glucose 6-phosphate, making C-6 into an active methylene, able to condense with the -CHO at C-1. Finally, the enzyme-bound NADH reconverts C-5 into the -CHOH- form.
CAS REGISTRY NUMBER
COMMENTARY
9032-95-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-Glucose 6-phosphate
?
-
the enzyme catalyzes the first committed step in the production of all inositol containing compounds, including phospholipids
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-
?
additional information
?
-
-
development of a continuous spectrophotometric method for measuring MIPS activity using a coupled assay that allows the rapid characterization of MIPS in a multiwell plate format, method validation, overview
-
-
?
D-Glucose 6-phosphate
L-myo-Inositol 1-phosphate
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-Glucose 6-phosphate
?
-
the enzyme catalyzes the first committed step in the production of all inositol containing compounds, including phospholipids
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
the activity of MIPS is moderately enhanced by the addition Mg2+ and is not enhanced by other divalent metal ions, such as Zn2+ and Mn2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.67 - 0.72
D-glucose 6-phosphate
-
pH 7.5, 30°C, dependent on the assay method, presence or absence of Mg2+
additional information
additional information
-
kinetics, effects of different divalent metal ions, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0817
D-glucose 6-phosphate
-
pH 7.5, 30°C, periodate assay method, in absence of 1 mM Mg2+
0.102
D-glucose 6-phosphate
-
pH 7.5, 30°C, periodate assay method, in presence of 1 mM Mg2+
0.107
D-glucose 6-phosphate
-
pH 7.5, 30°C, IMP-coupled assay method, in presence of 1 mM Mg2+
0.3
D-glucose 6-phosphate
-
pH 7.5, 30°C, IMP-coupled assay method, in presence of 10 mM Mg2+
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.12
D-glucose 6-phosphate
-
pH 7.5, 30°C, periodate assay method, in absence of 1 mM Mg2+
0.14
D-glucose 6-phosphate
-
pH 7.5, 30°C, periodate assay method, in presence of 1 mM Mg2+
0.157
D-glucose 6-phosphate
-
pH 7.5, 30°C, IMP-coupled assay method, in presence of 1 mM Mg2+
0.233
D-glucose 6-phosphate
-
pH 7.5, 30°C, IMP-coupled assay method, in presence of 10 mM Mg2+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
AtMIPS3 is stress inducible in the shoot by heat stress, mannitol, ABA, cold and NaCl
additional information
constitutive accumulation of its transcript is seen in flower and developing silique tissues
isoenzyme AtMIPS2-1 is largely heat inducible at the various growth stages such as seedling, flower, developing silique and mature siliques
is induced by abiotic stresses in 2-week-old seedlings
isoenzyme AtMIPS2-1 is largely heat inducible at the various growth stages such as seedling, flower, developing silique and mature siliques
constitutive accumulation of its transcript is seen in flower and developing silique tissues
isoenzyme AtMIPS2-1 is largely heat inducible at the various growth stages such as seedling, flower, developing silique and mature siliques
additional information
-
isoform MIPS1 is expressed in most cell types and developmental stages while isoforms MIPS2 and MIPS3 are mainly restricted to vascular or related tissues
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
endomembrane function in embryo cells is impaired in the mips1mips3 double mutant. Mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant phenotypes, overview
metabolism
MIPS catalyzes the rate-limiting step in de novo synthesis of myo-inositol
malfunction
metabolism
physiological function
malfunction
-
loss in MIPS1 results in smaller plants with curly leaves and spontaneous production of lesions
malfunction
endomembrane function in embryo cells is impaired in the mips1mips3 double mutant. Mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant phenotypes, overview
malfunction
null mips1 mutants are defective in embryogenesis, cotyledon venation patterning, root growth, and root cap development. The mutant roots are also agravitropic and have reduced basipetal auxin transport. mips1 mutants have significantly reduced levels of major phosphatidylinositols and exhibit much slower rates of endocytosis, as well as altered PIN2 trafficking, phenotype, detailed overview
malfunction
-
overexpression in Arabidopsis provides significantly improved tolerance to salt stress during germination and seedling growth and development. Transgenics retain more chlorophyll and carotenoid by protecting the photosystem II. The low level of stress-induced cellular damage in the transgenics is clearly evident by lower accumulation of proline in comparison to wild-tpye
metabolism
MIPS catalyzes the rate-limiting step in de novo synthesis of myo-inositol
metabolism
myo-inositol-1-phosphate synthase is a conserved enzyme that catalyzes the first committed and rate-limiting step in inositol biosynthesis
metabolism
-
the reaction catalyzed by MIPS is the first step in the biosynthesis of inositol and inositol-containing molecules that serve important roles in both eukaryotes and prokaryotes
physiological function
-
isoform MIPS1, but not MIPS2 or MIPS3, is required for seed development, for physiological responses to salt and abscisic acid, and to suppress cell death
physiological function
-
MIPS is a pivotal biosynthetic enzyme in the myo-inositol pathway, including the synthesis of phytic acid
physiological function
MIPS1 is required for multiple developmental processes. It is critical for maintaining phosphatidylinositol levels and affects pattern formation in plants likely through regulation of auxin distribution. MIPS1 is required for embryo development and vascular patterning
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). INO1_ARATH
511
0
56515
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
-
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
construction of transgenic plants expressing GFP-tagged MIPS using Agrobacterium tumefaciens GV3101 transfection method, overview
additional information
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
additional information
-
single mips mutants show no obvious phenotypes, but mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant are embryo lethal, whereas the mips1 mips3 and mips1 mips2+/- double mutants has abnormal embryos. The double and triple mips mutants display abnormal expression patterns of DR5:GFP, an auxin-responsive fusion protein, and they have altered PIN1 subcellular localization. Also, membrane trafficking is affected in mips1 mips3, overview. overexpression of phosphatidylinositol synthase 2, which converts myoinositol to membrane phosphatidylinositol, largely rescued the cotyledon and endomembrane defects in mips1 mips3
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
MIPS genes mips1, mips2, and mips3, phylogenetic analysis, the three Arabidopsis thaliana MIPS genes show dynamic expression patterns during Arabidopsis embryo development. They all functionally rescue the yeast ino1 mutant, which is defective in yeast MIPS gene INO1
MIPS genes mips1, mips2, and mips3, phylogenetic analysis, the three Arabidopsis thaliana MIPS genes show dynamic expression patterns during Arabidopsis embryo development. They all functionally rescue the yeast ino1 mutant, which is defective in yeast MIPS gene INO1
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
AtMIPS2-1 is largely heat inducible at the various growth stages such as seedling, flower, developing silique and mature siliques
AtMIPS3 is stress inducible in the shoot by heat stress, mannitol, ABA, cold and NaCl
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ishitani, M.; Majumder, A.L.; Bornhouser, A.; Michalowski, C.B.; Jensen, R.G.; Bohnert, H.J.
Coordinate transcriptional induction of myo-inositol metabolism during environmental stress
Plant J.
9
537-548
1996
Arabidopsis thaliana, Mesembryanthemum crystallinum
Johnson, M.D.
The Arabidopsis thaliana myo-inositol 1-phosphate synthase (EC 5.5.1.4)
Plant Physiol.
105
1023-1024
1994
Arabidopsis thaliana
Majumder, A.L.; Johnson, M.D.; Henry, S.A.
1L-myo-Inositol-1-phosphate synthase
Biochim. Biophys. Acta
1348
245-256
1997
Acer pseudoplatanus, Arabidopsis thaliana, Bos taurus, Brassica napus, Saccharomyces cerevisiae, Candida albicans, Streptomyces sp., Citrus x paradisi, Entamoeba histolytica, Euglena gracilis, Hevea brasiliensis, Homo sapiens, Lemna gibba, Lilium longiflorum, Mesembryanthemum crystallinum, Neurospora crassa, Vigna radiata, Phaseolus vulgaris, Pinus ponderosa, Rattus norvegicus, Spirodela polyrhiza, Arthrospira platensis
Mitsuhashi, N.; Kondo, M.; Nakaune, S.; Ohnishi, M.; Hayashi, M.; Hara-Nishimura, I.; Richardson, A.; Fukaki, H.; Nishimura, M.; Mimura, T.
Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis
J. Exp. Bot.
59
3069-3076
2008
Arabidopsis thaliana
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (P42801), Arabidopsis thaliana (Q38862), Arabidopsis thaliana (Q9LX12)
Abid, G.; Silue, S.; Muhovski, Y.; Jacquemin, J.M.; Toussaint, A.; Baudoin, J.P.
Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development
Gene
439
1-10
2009
Arabidopsis thaliana, Archaeoglobus fulgidus, Brassica napus, Citrus x paradisi, Euglena gracilis, Glycine max, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Perilla frutescens, Vigna radiata, Phaseolus vulgaris, Pisum sativum, Sesamum indicum, Solanum tuberosum, Zea mays
Donahue, J.L.; Alford, S.R.; Torabinejad, J.; Kerwin, R.E.; Nourbakhsh, A.; Ray, W.K.; Hernick, M.; Huang, X.; Lyons, B.M.; Hein, P.P.; Gillaspy, G.E.
The Arabidopsis thaliana myo-inositol 1-phosphate synthase1 gene is required for myo-inositol synthesis and suppression of cell death
Plant Cell
22
888-903
2010
Arabidopsis thaliana
Huang, X.; Hernick, M.
A limitation of the continuous spectrophotometric assay for the measurement of myo-inositol-1-phosphate synthase activity
Anal. Biochem.
417
228-232
2011
Arabidopsis thaliana
Chen, H.; Xiong, L.
myo-Inositol-1-phosphate synthase is required for polar auxin transport and organ development
J. Biol. Chem.
285
24238-24247
2010
Arabidopsis thaliana (C4PW05), Arabidopsis thaliana Columbia-0 (C4PW05)
Luo, Y.; Qin, G.; Zhang, J.; Liang, Y.; Song, Y.; Zhao, M.; Tsuge, T.; Aoyama, T.; Liu, J.; Gu, H.; Qu, L.J.
D-myo-Inositol-3-phosphate affects phosphatidylinositol-mediated endomembrane function in Arabidopsis and is essential for auxin-regulated embryogenesis
Plant Cell
23
1352-1372
2011
Arabidopsis thaliana (F4IIN3), Arabidopsis thaliana (P42801), Arabidopsis thaliana (Q9LX12), Arabidopsis thaliana
Khurana, N.; Chauhan, H.; Khurana, P.
Expression analysis of a heat-inducible, myo-inositol-1-phosphate synthase (MIPS) gene from wheat and the alternatively spliced variants of rice and Arabidopsis
Plant Cell Rep.
31
237-251
2012
Arabidopsis thaliana, Arabidopsis thaliana (Q38862), Arabidopsis thaliana (Q9LX12), Oryza sativa, Oryza sativa (O64437), Triticum aestivum (G8HMZ0), Triticum aestivum (Q9S7U0), Triticum aestivum
Joshi, R.; Ramanarao, M.V.; Baisakh, N.
Arabidopsis plants constitutively overexpressing a myo-inositol 1-phosphate synthase gene (SaINO1) from the halophyte smooth cordgrass exhibits enhanced level of tolerance to salt stress
Plant Physiol. Biochem.
65
61-66
2013
Arabidopsis thaliana
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