Information on EC 5.5.1.2 - 3-Carboxy-cis,cis-muconate cycloisomerase

Word Map on EC 5.5.1.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
5.5.1.2
-
RECOMMENDED NAME
GeneOntology No.
3-Carboxy-cis,cis-muconate cycloisomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination/addition
-
-
intramolecular
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
protocatechuate degradation II (ortho-cleavage pathway)
-
-
4-hydroxymandelate degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate lyase (decyclizing)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-77-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain S1
Uniprot
Manually annotated by BRENDA team
strain S1
Uniprot
Manually annotated by BRENDA team
strain PAO1
-
-
Manually annotated by BRENDA team
strain A.3.12., ATCC 12633
-
-
Manually annotated by BRENDA team
strain 1CP
-
-
Manually annotated by BRENDA team
strain 1CP
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
inactivation of CMLE does not affect superficial tomato root colonization, but does inhibit in planta growth, as well as growth and conidial germination in the presence of certain phenolic compounds
physiological function
-
CMLE is required for pathogenicity
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-5-oxo-2-tetrahydrofurancarboxylic acid
L-alpha-hydroxyglutaric acid
show the reaction diagram
-
-
-
-
?
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
cis,cis-butadiene-1,2,4-tricarboxylate
show the reaction diagram
2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate
cis-Butadiene-1,2,4-tricarboxylate
show the reaction diagram
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
cis-cis-butadiene-1,2,4-tricarboxylate
show the reaction diagram
-
-
-
?
3-carboxy-cis,cis-muconate
4-carboxy-(S)-muconolactone
show the reaction diagram
-
-
-
-
r
3-sulfomuconate
4-sulfomuconolactone + H2O
show the reaction diagram
D-glucono-D-lactone
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
requires no metal cofactor for catalytic activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
citrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
Dicarboxylic acids
-
saturated dicarboxylic acids with the exception of oxalate, are less inhibitory as the molecular weight increases. Compounds with two cis-carboxyl groups are much more inhibitory than the corresponding trans-isomers
EDTA
-
the enzyme is partially sensitive to EDTA
isocitrate
noncompetitive, localization of binding pocket on the enzyme outside the active site
phosphate
-
-
Tricarboxylic acids
-
-
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.052 - 0.32
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
7.1 - 15.3
3-sulfomuconate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.92 - 1415
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
20.9 - 42.7
3-sulfomuconate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
-
pH 8.0, 30°C
additional information
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
pH 5.5: about 70% of maximal activity, pH 9.0: about 25% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
x * 37000, SDS-PAGE
190000
-
gel filtration
191000
-
high-speed equilibrium sedimentation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
-
x-ray crystallography
octamer
-
8 * 23000-25000, SDS-PAGE, equilibrium sedimentation of enzyme treated with 5 M guanidine-HCl and 0.01% v/v 2-mercaptoethanol
tetramer
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme at 2.2 A resolution. C-terminal domain present in all other members of fumarase II family is missing and enzyme contains an Arg residue as opposed to Trp in the active site. Activation of the carboxylic nucleophile by a hydrophobic environment is not required for lactonization
ammonium sulfate precipitation, Semet protein, determination of structure at 3.0 A resolution
-
SeMet protein, X-ray structure at 2.5 A is a seven-bladed beta propeller
-
characterization of two crystal forms
-
hanging-drop vapor-diffusion method, 2.6 A resolution, symmetric of hexagonal space group P6(5) with unit cell parameters: a = b = 231.52 A, c = 78.46 A, alpha = beta = 90°, gamma = 120°. There are four PpCMLE molecules, forming a homotetramer, in the asymmetric unit; hanging drop vapour diffusion method with 12-18% PEG8000, 5 mM dithiothreitol, and 40 mM sodium phosphate (pH 7.3)
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
stable
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, completely stable
20°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 50% loss of activity
4°C, 50 mM Tris/HCl, 100 mM NaCl, 0.5 mM dithiothreitol, 36 days, completely stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; DEAE-Sepharcel column chromatography
-
His-Trap nickel affinity column chromatography
-
recombinant protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning and genetic organization of the pca gene cluster
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli strain N4830
-
expression as His-tagged protein in Escherichia coli
expression in Escherichia coli
Show AA Sequence (1069 entries)
Please use the Sequence Search for a specific query.