Information on EC 5.5.1.16 - halimadienyl-diphosphate synthase

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The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY hide
5.5.1.16
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RECOMMENDED NAME
GeneOntology No.
halimadienyl-diphosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate = tuberculosinyl diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tuberculosinol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
halima-5,13-dien-15-yl-diphosphate lyase (decyclizing)
Requires Mg2+ for activity. This enzyme is found in pathogenic prokaryotes such as Mycobacterium tuberculosis but not in non-pathogens such as Mycobacterium smegmatis so may play a role in pathogenicity. The product of the reaction is subsequently dephosphorylated yielding tuberculosinol (halima-5,13-dien-15-ol).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain H37Rv
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Manually annotated by BRENDA team
strain H37Rv3377c
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(E,E,E)-geranylgeranyl diphosphate
halimadienyl diphosphate
show the reaction diagram
geranylgeranyl diphosphate
halima-5(6),13-dien-15-yl diphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(E,E,E)-geranylgeranyl diphosphate
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substrate inhibition
15-aza-14,15-dihydrogeranylgeranyl diphosphate
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15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
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Mg2+
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the enzyme displays a rapid loss of activity as the Mg2+ concentration is raised above 0.1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0016
(E,E,E)-geranylgeranyl diphosphate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
(E,E,E)-geranylgeranyl diphosphate
Mycobacterium tuberculosis
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018
(E,E,E)-geranylgeranyl diphosphate
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000002
15-aza-14,15-dihydrogeranylgeranyl diphosphate
Mycobacterium tuberculosis
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0.0000001
15-aza-14,15-dihydrogeranylgeranyl thiolodiphosphate
Mycobacterium tuberculosis
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-4
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freezing leads to loss of about 10% activity over the course of 1 week
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, less than 24 h, the enzyme retains essentially full activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
amylose resin column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain C41
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expression in Escherichia coli
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