Information on EC 5.5.1.14 - syn-copalyl-diphosphate synthase

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The expected taxonomic range for this enzyme is: Oryza sativa

EC NUMBER
COMMENTARY hide
5.5.1.14
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RECOMMENDED NAME
GeneOntology No.
syn-copalyl-diphosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate = 9alpha-copalyl diphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular lyase
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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diterpene phytoalexins precursors biosynthesis
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Diterpenoid biosynthesis
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diterpene phytoalexins precursors biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
9alpha-copalyl-diphosphate lyase (decyclizing)
Requires a divalent metal ion, preferably Mg2+, for activity. This class II terpene synthase produces syn-copalyl diphosphate, a precursor of several rice phytoalexins, including oryzalexin S and momilactones A and B. Phytoalexins are diterpenoid secondary metabolites that are involved in the defense mechanism of the plant, and are produced in response to pathogen attack through the perception of elicitor signal molecules such as chitin oligosaccharide, or after exposure to UV irradiation. The enzyme is constitutively expressed in the roots of plants where one of its products, momilactone B, acts as an allelochemical (a molecule released into the environment to suppress the growth of neighbouring plants). In other tissues the enzyme is upregulated by conditions that stimulate the biosynthesis of phytoalexins.
CAS REGISTRY NUMBER
COMMENTARY hide
173585-12-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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the OsCPS4 knockdown mutant cps4-tos is more susceptible to rice blast fungus than the wild-type, possibly due to lower levels of momilactones and oryzalexin S in the mutant
metabolism
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the syn-copalyl diphosphate synthase is responsible for phytoalexins momilactones and oryzalexin S biosynthesis
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
syn-copalyl diphosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate
syn-copalyl diphosphate
show the reaction diagram
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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deduced from the existence of a transit peptide sequence
Manually annotated by BRENDA team
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography using Glutathione Sepharose B
hydroxyapatite chromatography, only partially purified
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of the full-length preprotein including the transit peptid in Escherichia coli, protein alone and as a C-terminal fusion to glutathione-S-transferase. Additionally, a partial cDNA sequence resembling the mature native protein was expressed alone as a potential pseudomature protein
expression of the full-length protein as a fusion protein with glutathione-S-transferase at the N-terminus in Escherichia coli XL1-Blue
genotyping of the Tos17 insertion mutant
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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generation of an OsCPS4 knockdown mutant, cps4-tos. Because Tos17 is inserted into the third intron of OsCPS4, the mature OsCPS4 mRNA is detected in the cps4-tos mutant as well as the wild type, screening Tos17 mutant lines using PCR. Mature OsCPS4 transcript levels in the cps4-tos mutant are about one sixth those in the wild-type. The cps4-tos mutant is more susceptible to rice blast fungus than the wild-type, possibly due to lower levels of momilactones and oryzalexin S in the mutant