Information on EC 5.5.1.10 - alpha-pinene-oxide decyclase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.5.1.10
-
RECOMMENDED NAME
GeneOntology No.
alpha-pinene-oxide decyclase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-pinene oxide = (Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular lyase reaction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Limonene and pinene degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-pinene-oxide lyase (decyclizing)
Both rings of pinene are cleaved in the reaction.
CAS REGISTRY NUMBER
COMMENTARY hide
112692-50-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain P18.3
-
-
Manually annotated by BRENDA team
strain P18.3
-
-
Manually annotated by BRENDA team
strain NCIMB 11671, wild-type and mutant cultures
-
-
Manually annotated by BRENDA team
strain NCIMB 11671, wild-type and mutant cultures
-
-
Manually annotated by BRENDA team
strain CIP 107491
-
-
Manually annotated by BRENDA team
strain PF1
-
-
Manually annotated by BRENDA team
strain NCIB 10684, NCIB 11671
-
-
Manually annotated by BRENDA team
strain NCIB 10684, NCIB 11671
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-pinene oxide
(Z)-2-methyl-5-isopropylhexa-2,5-dienal
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(Z)-2-Methyl-5-isopropylhexa-2,5-dienal
-
irreversible inactivation
5,5'-dithiobis(2-nitrobenzoate)
-
-
beta-Pinene oxide
-
-
Carvone
-
-
limonene oxide
-
-
p-hydroxymercuribenzoate
-
-
Pinan-3-ol
-
-
Pinan-3-one
-
-
Pinane derivatives with substituent groups at carbon 3
-
-
Sodium arsenite
-
weak
Sulfhydryl reactive compounds
-
-
-
Terpene epoxides
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
alpha-pinene
-
pH 9.0, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250
alpha-pinene
Nocardia sp.
-
pH 9.0, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
crude extract
120
-
after 13fold purification
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
no significant variation in activity over this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000
-
x * 17000 + x * 22000, SDS-PAGE
22000
-
x * 17000 + x * 22000, SDS-PAGE
50000
-
ultracentrifugal analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation of purified enzyme is mainly due to its direct contact with the hexadecane/water interface leading to precipitation
-
retains activity after prolonged dialysis
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 10 days, 7% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephacel column chromatography and Superdex 75 gel filtration
-
strain P18.3
-