Information on EC 5.4.99.B4 - 23S rRNA pseudouridine(helix 69) synthase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
5.4.99.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
23S rRNA pseudouridine(helix 69) synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
23S rRNA(helix 69) uridine = 23S rRNA(helix 69) pseudouridine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
23S rRNA uridine(helix 69) uracil mutase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Bifidobacterium bifidum strain Yakult YIT 4007
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Manually annotated by BRENDA team
serovar typhimurium
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine
23S rRNA pseudouridine
show the reaction diagram
50S ribosomal subunit
?
show the reaction diagram
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?
50S rRNA uridine
50S rRNA pseudouridine
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine
23S rRNA pseudouridine
show the reaction diagram
additional information
?
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Gar1
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the activity of Cbf5 is enhanced by protein Gar1 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction)
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Nop10
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the activity of Cbf5 is enhanced by protein Nop10 (increases Cbf5's affinity for tRNA, and also directly enhances Cbf5's catalytic activity by increasing the kcat of the reaction)
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00098
50S ribosomal subunit
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His-tagged enzyme, in 50 mM Tris/HCl at pH 8.0, 100 mM NH4Cl, 10 mM MgCl2, and 1 mM dithiothreitol, at 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.033
50S ribosomal subunit
Escherichia coli
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His-tagged enzyme, in 50 mM Tris/HCl at pH 8.0, 100 mM NH4Cl, 10 mM MgCl2, and 1 mM dithiothreitol, at 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
33.5
50S ribosomal subunit
Escherichia coli
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His-tagged enzyme, in 50 mM Tris/HCl at pH 8.0, 100 mM NH4Cl, 10 mM MgCl2, and 1 mM dithiothreitol, at 37°C
41732
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-agarose column chromatography
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Ni2+ Sepharose column chromatography and Superdex 75 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta 2(DE3) cells
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