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Information on EC 5.4.99.B22 - multisite-specific tRNA pseudouridine synthase and Organism(s) Mus musculus and UniProt Accession Q9WU56

for references in articles please use BRENDA:EC5.4.99.B22
preliminary BRENDA-supplied EC number
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This record set is specific for:
Mus musculus
UNIPROT: Q9WU56 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
hide(3 overall reactions are displayed. Show all (9)>>)
tRNA uridine65
=
tRNA pseudouridine65
tRNA uridine26
=
tRNA pseudouridine26
tRNA uridine27
=
tRNA pseudouridine27
Synonyms
pus1p, pseudouridine synthase 1, mpus1p, rpusd4, hpus1p, scpus1p, sppus1p, tgpus1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
RNA:pseudouridine synthases 1
-
-
SYSTEMATIC NAME
IUBMB Comments
multisite-specific tRNA-uridine uracil mutase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
human pre-tRNASer(UGA) uridine27
human pre-tRNASer(UGA) pseudouridine27
show the reaction diagram
-
-
-
?
yeast tRNASer(UAU) uridine27
yeast tRNASer(UAU) pseudouridine27
show the reaction diagram
-
-
-
?
yeast tRNASer(UAU) uridine34
yeast tRNASer(UAU) pseudouridine34
show the reaction diagram
-
-
-
?
yeast tRNASer(UAU) uridine36
yeast tRNASer(UAU) pseudouridine36
show the reaction diagram
-
-
-
?
yeast tRNAVal(UAC) uridine27
yeast tRNAVal(UAC) pseudouridine27
show the reaction diagram
-
-
-
?
yeast tRNAVal(UAC) uridine32
yeast tRNAVal(UAC) pseudouridine32
show the reaction diagram
-
-
-
?
pre-tRNAIle(UAU) uridine27
pre-tRNAIle(UAU) pseudouridine27
show the reaction diagram
-
-
-
-
?
pre-tRNAIle(UAU) uridine30
pre-tRNAIle(UAU) pseudouridine30
show the reaction diagram
-
-
-
-
?
pre-tRNAIle(UAU) uridine34
pre-tRNAIle(UAU) pseudouridine34
show the reaction diagram
-
-
-
-
?
pre-tRNAIle(UAU) uridine36
pre-tRNAIle(UAU) pseudouridine36
show the reaction diagram
-
-
-
-
?
tRNAArg(ACG) uridine1
tRNAArg(ACG) pseudouridine1
show the reaction diagram
-
Pus1p can catalyze pseudouridine1 formation in the mature tRNAArg, the pre-tRNAArg, and its maturation intermediates
-
-
?
tRNATrp(CCA) uridine26
tRNATrp(CCA) pseudouridine26
show the reaction diagram
-
low activity
-
-
?
tRNATrp(CCA) uridine27
tRNATrp(CCA) pseudouridine27
show the reaction diagram
-
-
-
-
?
tRNATrp(CCA) uridine28
tRNATrp(CCA) pseudouridine28
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
Pus1p is a member of the TruA pseudouridine synthase family
malfunction
-
U2 snRNAs from Pus1 knockout mice show no changes in their modification patterns when compared to wild-type U2 snRNAs. In Pus1-knockout mice, Pus1p-dependent modification of tRNAs is missing. No differences between wild-type and mutant mice in their U2 snRNA modification patterns
physiological function
-
in vertebrate SCARNA8, stronger binding in the alternative configuration to position U2-Psi45 might facilitate efficient sliding to position U2-Psi44. Multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications
additional information
-
screening for other potential guide RNAs for pseudouridylation of position 43 in U2 snRNA, vertebrate box H/ACA RNAs for antisense elements specific to the U2 snRNA branch point recognition region. The SNORA71 5'-terminal pseudouridylation pocket can base pair with U2 snRNA, both positions 43 and 41 can be modified by base-pairing within the same pseudouridylation pocket
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PUS1_MOUSE
423
0
47502
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D112A
-
inactive mutant enzyme
additional information
-
generation of Pus1 knockout mice
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Saccharomyces cerevisiae
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chen, J.; Patton, J.R.
Cloning and characterization of a mammalian pseudouridine synthase
RNA
5
409-419
1999
Mus musculus (Q9WU56), Mus musculus
Manually annotated by BRENDA team
Behm-Ansmant, I.; Massenet, S.; Immel, F.; Patton, J.R.; Motorin, Y.; Branlant, C.
A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs
RNA
12
1583-1593
2006
Mus musculus, Saccharomyces cerevisiae (Q12211), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Deryusheva, S.; Gall, J.G.
Dual nature of pseudouridylation in U2 snRNA Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes
RNA
23
1060-1067
2017
Caenorhabditis elegans, Drosophila melanogaster, Drosophila melanogaster yw, Mus musculus, Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741, Schizosaccharomyces pombe, Schizosaccharomyces pombe 972, Xenopus tropicalis
Manually annotated by BRENDA team