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Synonyms
pus1p, mpus1p, pseudouridine synthase 1, rpusd4, hpus1p, scpus1p, sppus1p, tgpus1,
more
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RPL11a mRNA uridine68
RPL11a mRNA pseudouridine68
the Pus1 enzyme is necessary and sufficient for pseudouridylation of RPL11a mRNA
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tRNAArg(ACG) uridine1
tRNAArg(ACG) pseudouridine1
Pus1p can catalyze pseudouridine1 formation in the mature tRNAArg, the pre-tRNAArg, and its maturation intermediates
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tRNAIle uridine27
tRNAIle pseudouridine27
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tRNAIle(UAU) uridine27
tRNAIle(UAU) pseudouridine27
intron-independent reaction
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tRNAIle(UAU) uridine34
tRNAIle(UAU) pseudouridine34
tRNAIle(UAU) uridine36
tRNAIle(UAU) pseudouridine36
tRNATrp(CCA) uridine26
tRNATrp(CCA) pseudouridine26
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tRNATrp(CCA) uridine27
tRNATrp(CCA) pseudouridine27
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tRNATrp(CCA) uridine28
tRNATrp(CCA) pseudouridine28
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tRNATrp(CCA) uridine65
tRNATrp(CCA) pseudouridine65
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tRNATrp(CCA) uridine67
tRNATrp(CCA) pseudouridine67
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tRNAVal uridine27
tRNAVal pseudouridine27
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KAR2 mRNA uridine
KAR2 mRNA pseudouridine
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TEF1 mRNA uridine239
TEF1 mRNA pseudouridine239
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tRNA uridine13
tRNA pseudouridine13
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additional information
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tRNAIle(UAU) uridine34
tRNAIle(UAU) pseudouridine34
intron-dependent reaction
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tRNAIle(UAU) uridine34
tRNAIle(UAU) pseudouridine34
reaction is strictly intron-dependent
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tRNAIle(UAU) uridine36
tRNAIle(UAU) pseudouridine36
intron-dependent reaction
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tRNAIle(UAU) uridine36
tRNAIle(UAU) pseudouridine36
reaction is strictly intron-dependent
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additional information
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Pus1p catalyzes the formation of pseudouridines in several positions and in a number of different tRNA species
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additional information
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Pus1p catalyzes the formation of pseudouridines in several positions and in a number of different tRNA species
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additional information
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Pus1 from Saccharomyces cerevisiae is a multisite-specific enzyme that catalyses the formation of pseudouridine residues at different positions in several tRNA transcripts
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additional information
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for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus1-mediated Psi sites at positions 26-28 in several tRNAs, Pus7-dependent sites at position 13 of glutamate tRNA, Pus9-dependent site in mitochondrial aspartate tRNA at position 32, and Deg1-dependent sites at positions 37-38 in several tRNAs. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions
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additional information
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Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44
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additional information
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dual nature of pseudouridylation in U2 snRNA, Pus1p-dependent and Pus1p-independent activities. Position 44 in yeast U2 snRNA (equivalent to position 43 in vertebrates) is a genuine substrate for Pus1p. Besides U2 and U6 snRNAs in Saccharomyces cerevisiae, the enzyme modifies uridines in many different tRNAs. Substrate specificity analysis and comparison of different species, two types of pseudouridine synthase that catalyze the formation of U2-?43 (or 44) in yeasts, Drosophila, and vertebrates
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RPL11a mRNA uridine68
RPL11a mRNA pseudouridine68
the Pus1 enzyme is necessary and sufficient for pseudouridylation of RPL11a mRNA
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?
KAR2 mRNA uridine
KAR2 mRNA pseudouridine
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TEF1 mRNA uridine239
TEF1 mRNA pseudouridine239
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tRNA uridine13
tRNA pseudouridine13
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additional information
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additional information
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Pus1p catalyzes the formation of pseudouridines in several positions and in a number of different tRNA species
-
-
?
additional information
?
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Pus1p catalyzes the formation of pseudouridines in several positions and in a number of different tRNA species
-
-
?
additional information
?
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for tRNA and Pus7, many known sites and their associated modifiers are identified, including Pus1-mediated Psi sites at positions 26-28 in several tRNAs, Pus7-dependent sites at position 13 of glutamate tRNA, Pus9-dependent site in mitochondrial aspartate tRNA at position 32, and Deg1-dependent sites at positions 37-38 in several tRNAs. Pseudouridylation in ncRNAs at sites of inter or intra-molecular interactions
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?
additional information
?
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Pus1p is the only pseudouridine synthase in Saccharomyces cerevisiae that is active on U2 snRNA at position 44. Yeast U2 snRNA is normally pseudouridylated at positions 35, 42, and 44
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?
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malfunction
the disruption of the Pus1 gene confers no obvious phenotype
metabolism
the Pus1 enzyme is necessary and sufficient for pseudouridylation of RPL11a mRNA
evolution
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Pus1p is a member of the TruA pseudouridine synthase family
additional information
transcriptome-wide quantitative mapping of Psi, method development using Psi-seq, relying on the unique stability of N3-[N-cyclohexyl-N'-beta-(4-methylmorpholinium)ethylcarbodiimide-Psi] (N3-CMC-Psi) to alkaline hydrolysis, and the ability of N3-CMC-Psi to terminate reverse transcription. For rRNA, 24/24 Cbf5-dependent sites are known targets of pseudouridylation, 8/9 associations between snoRNAs and rRNA sites are known, as is the identified Pus5-mediated Psi site in mitochondrial 21S rRNA. One snR3-dependent site at position 2140 on 25S rRNA is not specificated, detailed overview. Cbf5-dependent Psi sites in mRNAs and snoRNAs are likely snoRNA-guided
physiological function
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multiple guide RNAs are typically assigned to the most important modified positions. Pseudouridines at the branch point recognition region of U2 snRNA represent such functionally crucial modifications
physiological function
the most abundant RNA modification is pseudouridine (Psi), Psi is ubiquitous in diverse RNAs, and dynamic in mRNA. Pseudouridylation profiles and mechanisms. Pus7-dependent induction of pseudouridylation during heat shock
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Motorin, Y.; Keith, G.; Simon, C; Foiret, D.; Simos, G.; Hurt, E.; Grosjean, H.
The yeast tRNA: pseudouridine synthase Pus1p displays a multisite substrate specificity
RNA
4
856-869
1998
Saccharomyces cerevisiae (Q12211), Saccharomyces cerevisiae
brenda
Arluison, V.; Hountondji, C.; Robert, B.; Grosjean, H.
Transfer RNA-Pseudouridine Synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition
Biochemistry
37
7268-7276
1998
Saccharomyces cerevisiae (Q12211)
brenda
Arluison, V.; Buckle, M.; Grosjean, H.
Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA
J. Mol. Biol.
289
491-502
1999
Saccharomyces cerevisiae (Q12211)
brenda
Arluison, V.; Batelier, G.; Ris-Kautt, M.; Grosjean, H.
RNA: pseudouridine synthase pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA Phe
Biochimie
81
751-756
1999
Saccharomyces cerevisiae (Q12211)
brenda
Behm-Ansmant, I.; Massenet, S.; Immel, F.; Patton, J.R.; Motorin, Y.; Branlant, C.
A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs
RNA
12
1583-1593
2006
Mus musculus, Saccharomyces cerevisiae (Q12211), Saccharomyces cerevisiae
brenda
Simos, G.; Tekotte, H.; Grosjean, H.; Segref, A.; Sharma, K.; Tollervey, D.; Hurt, E.C.
Nuclear pore proteins are involved in the biogenesis of functional tRNA
EMBO J.
15
2270-2284
1996
Saccharomyces cerevisiae (Q12211)
brenda
Schwartz, S.; Bernstein, D.A.; Mumbach, M.R.; Jovanovic, M.; Herbst, R.H.; Leon-Ricardo, B.X.; Engreitz, J.M.; Guttman, M.; Satija, R.; Lander, E.S.; Fink, G.; Regev, A.
Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA
Cell
159
148-162
2014
Saccharomyces cerevisiae (Q08647)
brenda
Lovejoy, A.F.; Riordan, D.P.; Brown, P.O.
Transcriptome-wide mapping of pseudouridines pseudouridine synthases modify specific mRNAs in S. cerevisiae
PLoS ONE
9
e110799
2014
Saccharomyces cerevisiae (P48567), Saccharomyces cerevisiae (P53294), Saccharomyces cerevisiae (Q12211), Saccharomyces cerevisiae ATCC 204508 (P48567), Saccharomyces cerevisiae ATCC 204508 (P53294), Saccharomyces cerevisiae ATCC 204508 (Q12211)
brenda
Deryusheva, S.; Gall, J.G.
Dual nature of pseudouridylation in U2 snRNA Pus1p-dependent and Pus1p-independent activities in yeasts and higher eukaryotes
RNA
23
1060-1067
2017
Caenorhabditis elegans, Drosophila melanogaster, Drosophila melanogaster yw, Mus musculus, Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741, Schizosaccharomyces pombe, Schizosaccharomyces pombe 972, Xenopus tropicalis
brenda