Information on EC 5.4.99.B2 - multifunctional triterpene synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.99.B2
preliminary BRENDA-suppliled EC number
RECOMMENDED NAME
GeneOntology No.
multifunctional triterpene synthase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cultivar Royal Gala
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Manually annotated by BRENDA team
cultivars MicroTom, M82, and Ailsa Craig
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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CrAS is multifunctional 2,3-oxidosqualene cyclase (OSC) producing alpha- and beta-amyrin in a ratio of 2.5:1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2,3-oxidosqualene
alpha-amyrin + beta-amyrin
show the reaction diagram
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OSC1 produces a 5:1 ratio of alpha-amyrin to beta-amyrin
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?
(S)-2,3-oxidosqualene
delta-amyrin + beta-amyrin + alpha-amyrin + multiflorenol + psi-taraxasterol + taraxasterol + ?
show the reaction diagram
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isoform SITT1 produces 48% delta-amyrin, 13% beta-amyrin, 18% alpha-amyrin, and 3-7% each of multiflorenol, psi-taraxasterol, taraxasterol, and one unidentified triterpene alcohol isomer
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?
alpha-amyrin + beta-amyrin (?)
(S)-2,3-oxidosqualene
show the reaction diagram
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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highest expression
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
87730
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calculated from cDNA
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
thin-layer chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana benthamiana leaves and in Pichia methanolica
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expressed in Saccharomyces cerevisiae mutant GIL77
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expressed in yeast
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
high expression in leaves
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