Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 5.4.99.8 - cycloartenol synthase and Organism(s) Arabidopsis thaliana and UniProt Accession P38605

for references in articles please use BRENDA:EC5.4.99.8
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.8 cycloartenol synthase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: P38605 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
cycloartenol synthase, sgcas, ntcas, cacys, wscas, cycloartenol synthase 1, nbcas, ldcas, 2,3-oxidosqualene:cycloartenol cyclase, (s)-2,3-epoxysqualene mutase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cycloartenol synthase
-
2,3-Epoxysqualene cycloartenol-cyclase
-
-
-
-
2,3-epoxysqualene--cycloartenol cyclase
-
-
-
-
2,3-Epoxysqualene-cycloartenol cyclase
-
-
-
-
2,3-Oxidosqualene cycloartenol cyclase
-
-
-
-
2,3-Oxidosqualene-cycloartenol cyclase
-
-
-
-
2,3-Oxidosqualene:cycloartenol cyclase
-
-
-
-
Cyclase, 2,3-oxidosqualene-cycloartenol
-
-
-
-
cycloartenol synthase
-
-
cycloartenol synthase 1
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(S)-2,3-Epoxysqualene mutase (cyclizing, cycloartenol-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-25-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-2,3-oxidosqualene
cycloartenol
show the reaction diagram
-
-
-
?
(S)-2,3-Epoxysqualene
Cycloartenol
show the reaction diagram
(S)-2,3-oxidosqualene
cycloartenol
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2,3-oxidosqualene
cycloartenol
show the reaction diagram
-
-
-
?
(S)-2,3-Epoxysqualene
Cycloartenol
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAS1_ARATH
759
0
86033
Swiss-Prot
other Location (Reliability: 5)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H477N
produces 88% lanosterol and 12% parkeol
H477Q
produces 22% lanosterol, 73% parkeol, and 5% 9-beta-delta-7-lanosterol
I481V
produces 54% cycloartenol, 25% lanosterol, and 21% parkeol
Y410T
produces 65% lanosterol, 2% parkeol, and 33% 9-beta-delta-7-lanosterol
Y410T/H477N/I481V
produces 78% lanosterol and 22% 9-beta-delta-7-lanosterol
Y410T/H477Q/I481V
produces 78% lanosterol and 22% 9-beta-delta-7-lanosterol
Y410T/I481V
produces 78% lanosterol, less than 1% parkeol, and 22% 9-beta-delta-7-lanosterol
H477N
H477N/I481V
-
produces 99% lanosterol and 1% parkeol
H477Q
H477Q/I481V
-
produces 94% lanosterol and 6% parkeol
I481A
-
production of lanosterol, cycloartenol, parkeol, acilleol A and camelliol C
I481F
-
inactive
I481G
-
production of lanosterol, cycloartenol, parkeol, acilleol A and camelliol C
I481L
-
production of cycloartenol and parkeol
I481V
-
produces 54% cycloartenol, 25% lanosterol, and 21% parkeol
Y410T
-
produces 65% lanosterol, 2% parkeol, and 33% 9-beta-delta-7-lanosterol
Y410T/H477N/I481V
-
produces 78% lanosterol and 22% 9-beta-delta-7-lanosterol
Y410T/H477Q/I481V
-
produces 78% lanosterol and 22% 9-beta-delta-7-lanosterol
Y410T/I481V
Y532H
-
mutated enzyme produces a mixture of parkeol, achilleol and lanosterol instead of cycloartenol
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
mutants are expressed in Saccharomyces cerevisiae strain LHY4
expression in Escherichia coli
-
expression in Saccharomyces cerevisiae
-
expression in yeast
-
sequence comparisons of lanosterol synthases and cycloartenol synthases from several organisms
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Corey, E.J.; Matsuda, S.P.T.; Bartel, B.
Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen
Proc. Natl. Acad. Sci. USA
90
11628-11632
1993
Arabidopsis thaliana
Manually annotated by BRENDA team
Matsuda, S.P.; Darr, L.B.; Hart, E.A.; Herrera, J.B.; McCann, K.E.; Meyer, M.M.; Pang, J.; Schepmann, H.G.
Steric bulk at cycloartenol synthase position 481 influences cyclization and deprotonation
Org. Lett.
2
2261-2263
2000
Arabidopsis thaliana
Manually annotated by BRENDA team
Segura, M.J.; Lodeiro, S.; Meyer, M.M.; Patel, A.J.; Matsuda, S.P.
Directed evolution experiments reveal mutations at cycloartenol synthase residue His477 that dramatically alter catalysis
Org. Lett.
4
4459-4462
2002
Arabidopsis thaliana
Manually annotated by BRENDA team
Lodeiro, S.; Segura, M.J.R.; Stahl, M.; Schulz-Gasch, T.; Matsuda, S.P.T.
Oxidosqualene cyclase second-sphere residues profoundly influence the product profile
ChemBioChem
5
1581-1585
2004
Arabidopsis thaliana (P38605)
Manually annotated by BRENDA team
Lodeiro, S.; Schulz-Gasch, T.; Matsuda, S.P.T.
Enzyme redesign: two mutations cooperate to convert cycloartenol synthase into an accurate lanosterol synthase
J. Am. Chem. Soc.
127
14132-14133
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Kolesnikova, M.D.; Xiong, Q.; Lodeiro, S.; Hua, L.; Matsuda, S.P.
Lanosterol biosynthesis in plants
Arch. Biochem. Biophys.
447
87-95
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Babiychuk, E.; Bouvier-Nave, P.; Compagnon, V.; Suzuki, M.; Muranaka, T.; Van Montagu, M.; Kushnir, S.; Schaller, H.
Allelic mutant series reveal distinct functions for Arabidopsis cycloartenol synthase 1 in cell viability and plastid biogenesis
Proc. Natl. Acad. Sci. USA
105
3163-3168
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Ohyama, K.; Suzuki, M.; Kikuchi, J.; Saito, K.; Muranaka, T.
Dual biosynthetic pathways to phytosterol via cycloartenol and lanosterol in Arabidopsis
Proc. Natl. Acad. Sci. USA
106
725-730
2009
Arabidopsis thaliana
Manually annotated by BRENDA team