Information on EC 5.4.99.61 - precorrin-8X methylmutase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
5.4.99.61
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RECOMMENDED NAME
GeneOntology No.
precorrin-8X methylmutase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Precorrin-8X = hydrogenobyrinate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)
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Metabolic pathways
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Porphyrin and chlorophyll metabolism
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vitamin B12 metabolism
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SYSTEMATIC NAME
IUBMB Comments
precorrin-8X 11,12-methylmutase
The enzyme catalyses the the conversion of precorrin-8X to hydrogenobyrinate by methyl rearrangement, a step in the aerobic (late cobalt insertion) pathway of adenosylcobalamin biosynthesis. The equivalent enzyme in the anaerobic pathway is EC 5.4.99.60, precorrin-8 methylmutase.
CAS REGISTRY NUMBER
COMMENTARY hide
138238-71-8
gene cbiC precorrin-8x isomerase /gene cobH precorrin-8x isomerase
181967-26-0
isomerase, precorrin (Methanococcus jannaschii gene MJ0930) / genBank U67537-derived protein GI 1499765
198584-26-8
isomerase, precorrin (Methanobacterium thermoautothrophicum strain DELTAH gene MTH227) /genBank AE00809-derived protein GI 2621284
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain SC510 Rifr(pXL1149)
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Precorrin-8X
?
show the reaction diagram
Precorrin-8X
Hydrogenobyrinate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Precorrin-8X
?
show the reaction diagram
Precorrin-8X
Hydrogenobyrinate
show the reaction diagram
P21638
involved in the aerobic pathway to vitamin B12
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?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hydrogenobyrinic acid
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competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00091
Hydrogenobyrinate
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0.91
precorrin-8X
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00142
precorrin-8X
Pseudomonas denitrificans
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00017
Hydrogenobyrinate
product inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22000
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x * 22000, calculation from nucleotide sequence
22013
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2 * 22013, calculation from nucleotide sequence
22060
gel filtration, native-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 22000, calculation from nucleotide sequence
dimer
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2 * 22013, calculation from nucleotide sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
apo form and complexed with hydrogenobirinate, hanging drop vapor diffusion with equal volumes of concentrated protein, i.e. 10 mg/ml, and a precipitant solution containing 25%-32.5% polyethylene glycol 4000, 200 mM MgCl2, 100 mM Tris-HCl, pH 8.5, crystals diffract to 2.1 A and 2.6 A resolution
2.1 A crystal structure
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Escherichia coli
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