Information on EC 5.4.99.60 - cobalt-precorrin-8 methylmutase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.99.60
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RECOMMENDED NAME
GeneOntology No.
cobalt-precorrin-8 methylmutase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cobalt-precorrin-8 = cobyrinate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)
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Metabolic pathways
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Porphyrin and chlorophyll metabolism
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vitamin B12 metabolism
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SYSTEMATIC NAME
IUBMB Comments
precorrin-8 11,12-methylmutase
The enzyme catalyses the the conversion of cobalt-precorrin-8 to cobyrinate by methyl rearrangement, a step in the anaerobic (early cobalt insertion) pathway of adenosylcobalamin biosynthesis. The equivalent enzyme in the aerobic pathway is EC 5.4.99.61, precorrin-8X methylmutase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cobalt-precorrin-8
cobyrinate
show the reaction diagram
Precorrin-8X
?
show the reaction diagram
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the enzyme is involved in the cobalamin biosynthetic pathway
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Precorrin-8X
Hydrogenobyrinate
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cobalt-precorrin-8
cobyrinate
show the reaction diagram
Precorrin-8X
?
show the reaction diagram
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the enzyme is involved in the cobalamin biosynthetic pathway
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
x-ray crystallography
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using either 10% (v/v) PEG6000, 2.0 M sodium chloride or 20% (v/v) ethanol, 0.1 M Tris, pH 8.5; two crystal forms of space groups P42212 and P3121, diffracting to 3.0 and 2.3 A resolution, resp.
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-affinity column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells; expression in Escherichia coli as His-tagged protein
homologously overproduced enzyme
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