Information on EC 5.4.99.59 - dTDP-fucopyranose mutase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
5.4.99.59
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RECOMMENDED NAME
GeneOntology No.
dTDP-fucopyranose mutase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-alpha-D-fucopyranose = dTDP-alpha-D-fucofuranose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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dTDP-D-beta-fucofuranose biosynthesis
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Polyketide sugar unit biosynthesis
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dTDPLrhamnose biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dTDP-alpha-D-fucopyranose furanomutase
The enzyme is involved in the biosynthesis of the Escherichia coli O52 O antigen.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-alpha-D-fucopyranose
dTDP-beta-D-fuco-(1->4)-furanose
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-alpha-D-fucopyranose
dTDP-beta-D-fuco-(1->4)-furanose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Fcf2 is an divalent cation-independent enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
5 mM, complete inhibition
Mn2+
5 mM, 43% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.43
dTDP-alpha-D-fucopyranose
pH 7.4, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.056
dTDP-alpha-D-fucopyranose
Escherichia coli
Q6E7F1
pH 7.4, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016
dTDP-alpha-D-fucopyranose
Escherichia coli
Q6E7F1
pH 7.4, 37C
9565
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 50
4C: 17% of maximal activity, 50C: 49% of maximal activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, in 50% glycerol, stable for at least 2 months
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His6-tagged fusion protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli BL21