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Information on EC 5.4.99.44 - mitochondrial tRNA pseudouridine27/28 synthase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P53167

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IUBMB Comments
The mitochondrial enzyme Pus2p is specific for position 27 or 28 in mitochondrial tRNA .
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P53167
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
mitochondrial tRNA uridine27/28
=
mitochondrial tRNA pseudouridine27/28
Synonyms
pus2p, mitochondrial-specific trna:psi-synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mitochondrial-specific tRNA:Psi-synthase
-
tRNA:PSI27/28-synthase
-
SYSTEMATIC NAME
IUBMB Comments
mitochondrial tRNA-uridine27/28 uracil mutase
The mitochondrial enzyme Pus2p is specific for position 27 or 28 in mitochondrial tRNA [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mitochondrial tRNAArg(UCU) uridine27
mitochondrial tRNAArg(UCU) pseudouridine27
show the reaction diagram
Pus2p is specifically dedicated to mitochondrial tRNA modification, the corresponding modification in cytoplasmic tRNAs is catalyzed by another protein (Pus1p). Pus2p does not catalyze pseudouridylation at position 72. Other pseudouridines in cytoplasmic yeast tRNA normally formed by Pus1p (positions 1, 34, 36, 65, 67) are absent in the mitochondrial compartment
-
-
?
mitochondrial tRNALys(UUU) uridine28
mitochondrial tRNALys(UUU) pseudouridine28
show the reaction diagram
Pus2p is specifically dedicated to mitochondrial tRNA modification, the corresponding modification in cytoplasmic tRNAs is catalyzed by another protein (Pus1p). Pus2p does not catalyze pseudouridylation at position 72. Other pseudouridines in cytoplasmic yeast tRNA normally formed by Pus1p (positions 1, 34, 36, 65, 67) are absent in the mitochondrial compartment
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
mitochondrial tRNAArg(UCU) uridine27
mitochondrial tRNAArg(UCU) pseudouridine27
show the reaction diagram
Pus2p is specifically dedicated to mitochondrial tRNA modification, the corresponding modification in cytoplasmic tRNAs is catalyzed by another protein (Pus1p). Pus2p does not catalyze pseudouridylation at position 72. Other pseudouridines in cytoplasmic yeast tRNA normally formed by Pus1p (positions 1, 34, 36, 65, 67) are absent in the mitochondrial compartment
-
-
?
mitochondrial tRNALys(UUU) uridine28
mitochondrial tRNALys(UUU) pseudouridine28
show the reaction diagram
Pus2p is specifically dedicated to mitochondrial tRNA modification, the corresponding modification in cytoplasmic tRNAs is catalyzed by another protein (Pus1p). Pus2p does not catalyze pseudouridylation at position 72. Other pseudouridines in cytoplasmic yeast tRNA normally formed by Pus1p (positions 1, 34, 36, 65, 67) are absent in the mitochondrial compartment
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
efficient and rapid targeting of Pus2p in yeast mitochondria
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
disruption of the PUS2 gene leads to the absence of pseudouridine27 residues in the mitochondrial tRNAArg(UCU) and pseudouridine28 in the mitochondrial tRNALys(UUU)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Behm-Ansmant, I.; Branlant, C.; Motorin, Y.
The Saccharomyces cerevisiae Pus2 protein encoded by YGL063w ORF is a mitochondrial tRNA:PSI27/28-synthase
RNA
13
1641-1647
2007
Saccharomyces cerevisiae (P53167), Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742 (P53167)
Manually annotated by BRENDA team