Information on EC 5.4.99.42 - tRNA pseudouridine31 synthase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY
5.4.99.42
-
RECOMMENDED NAME
GeneOntology No.
tRNA pseudouridine31 synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
tRNA uridine31 = tRNA pseudouridine31
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine31 uracil mutase
The enzyme specifically acts on uridine31 in tRNA.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pseudouridylate synthase 6
P53294
-
tRNA:Psi31-synthase
P53294
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
P53294, -
the deletion mutant strain DELTAYGR169c shows no detectable growth phenotype. The only difference in pseudouridine formation in stable cellular RNAs is the absence of pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs. Complementation of the DELTAYGR169c strain by a plasmid bearing the wild-type YGR169c ORF restores pseudouridine31 formation in tRNA
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
tRNA uridine31
tRNA pseudouridine31
show the reaction diagram
P53294, -
the enzyme is responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31
-
-
?
tRNA uridine31
tRNA pseudouridine31
show the reaction diagram
P53294, -
cytoplasmic and mitochondrial tRNA(Met), mitochondrial tRNA(Lys) and tRNA(Ser). Recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates. The enzyme does not catalyse the pseudouridylation of uridine31
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine31
tRNA pseudouridine31
show the reaction diagram
P53294, -
the enzyme is responsible for modification of cytoplasmic and mitochondrial tRNAs at position 31
-
-
?
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant His6-tagged Ygr169 protein produced in Escherichia coli is capable of forming pseudouridine31 in vitro using tRNAs extracted from the DELTAYGR169c yeast cells as substrates
P53294, -
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D168A
P53294, -
mutation abolishes tRNA pseudouridine31 synthase activity