Information on EC 5.4.99.29 - 23S rRNA pseudouridine746 synthase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
5.4.99.29
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RECOMMENDED NAME
GeneOntology No.
23S rRNA pseudouridine746 synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
23S rRNA uridine746 = 23S rRNA pseudouridine746
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
23S rRNA-uridine746 uracil mutase
RluA is the sole protein responsible for the in vivo formation of 23S RNA pseudouridine746 [2]. The dual-specificity enzyme also catalyses the formation of uridine32 in tRNA [3]. cf. EC 5.4.99.28 (tRNA pseudouridine32 synthase).
CAS REGISTRY NUMBER
COMMENTARY hide
61506-89-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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the rluA deletion mutant fails to form either 23S RNA pseudouridine746 or tRNA pseudouridine32. Replacement of rluA in trans on a rescue plasmid restores both pseudouridines. There is no difference in exponential growth rate between wild-type and MG1655(rluA-) either in rich or minimal medium at 24, 37, or 42°C, but when both strains are grown together, a strong selection against the deletion strain is observed
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine746
23S rRNA pseudouridine746
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine746
23S rRNA pseudouridine746
show the reaction diagram
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RluA is the sole synthase for formation of pseudouridine746 in 23S rRNA
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?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24432
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x * 24432, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 24432, calculated from sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
vapor diffusion method. 2.05 A resolution structure of RluA bound to a substrate RNA comprising the anticodon stem loop of tRNAPhe reveals that enzyme binding induces a dramatic reorganization of the RNA
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
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Tm-value for mutant enzyme P29L
38
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Tm-value for mutant enzyme P29G is 37.9°C
45
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Tm-value for wild-type enzyme is 44.9°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D64N
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mutation blocks synthesis of pseudouridine746, mutant rluA constructs produces a stable protein
D64T
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mutation blocks synthesis of pseudouridine746, mutant rluA constructs produces a stable protein
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