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Information on EC 5.4.99.12 - tRNA pseudouridine38-40 synthase and Organism(s) Mus musculus and UniProt Accession Q9JI38

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.12 tRNA pseudouridine38-40 synthase
IUBMB Comments
The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures .
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This record set is specific for:
Mus musculus
UNIPROT: Q9JI38
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
tRNA uridine38-40
=
tRNA pseudouridine38-40
Synonyms
trna pseudouridine synthase i, mpus3p, pus3p, deg1p, pseudouridine synthase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pseudouridine synthase 3
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-uridine38-40 uracil mutase
The uridylate residues at positions 38, 39 and 40 of nearly all tRNAs are isomerized to pseudouridine. TruA specifically modifies uridines at positions 38, 39, and/or 40 in the anticodon stem loop of tRNAs with highly divergent sequences and structures [1].
CAS REGISTRY NUMBER
COMMENTARY hide
430429-15-5
-
61506-89-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
human tRNALeu uridine 38 uridine39
human tRNALeu pseudouridine 38 pseudouridine39
show the reaction diagram
-
psuedouridine formation in position 39 is clearly preferred over position 38
-
?
human tRNALys3
?
show the reaction diagram
-
-
-
?
human tRNASer uridine39
human tRNASer pseudouridine39
show the reaction diagram
-
-
-
?
tRNA uridine39
tRNA pseudouridine39
show the reaction diagram
with tRNA substrates from both yeast and humans, uridines at position 39 are modified to pseudouridine. In a tRNA substrate with a uridine at position 38 (human tRNALeu), there is very slight formation of pseudouridine at that position after incubation with mPus3p
-
-
?
yeast tRNAPhe
?
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tRNA uridine39
tRNA pseudouridine39
show the reaction diagram
with tRNA substrates from both yeast and humans, uridines at position 39 are modified to pseudouridine. In a tRNA substrate with a uridine at position 38 (human tRNALeu), there is very slight formation of pseudouridine at that position after incubation with mPus3p
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PUS3_MOUSE
481
0
55546
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55552
x * 55552, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55552, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chen, J.; Patton, J.R.
Pseudouridine synthase 3 from mouse modifies the anticodon loop of tRNA
Biochemistry
39
12723-12730
2000
Mus musculus (Q9JI38), Mus musculus
Manually annotated by BRENDA team