Binds Fe2+. The enzyme from mammals accepts a range of hydroperoxyicosatetraenoates producing one or several different hydroxyepoxyicosatrienoates. The human enzyme has highest activity with (12R)-HPETE producing (5Z,8R,9E,11R,12R,14Z)-8-hydroxy-11,12-epoxyicosa-5,9,14-trienoate, followed by (12S)-HPETE producing (5Z,8Z,10R,11S,12S,14Z)-10-hydroxy-11,12-epoxyicosa-5,8,14-trienoate and (5Z,8R,9E,11S,12S,14Z)-8-hydroxy-11,12-epoxyicosa-5,9,14-trienoate . The mouse enzyme has highest activity with (8S)-HPETE, producing (5Z,8S,9S,10R,11Z,14Z)-10-hydroxy-8,9-epoxyicosa-5,11,14-trienoate . The enzymes also have the activity of EC 4.2.1.152, hydroperoxy icosatetraenoate dehydratase.
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SYSTEMATIC NAME
IUBMB Comments
hydroperoxyicosatetraenoate hydroxymutase
Binds Fe2+. The enzyme from mammals accepts a range of hydroperoxyicosatetraenoates producing one or several different hydroxyepoxyicosatrienoates. The human enzyme has highest activity with (12R)-HPETE producing (5Z,8R,9E,11R,12R,14Z)-8-hydroxy-11,12-epoxyicosa-5,9,14-trienoate, followed by (12S)-HPETE producing (5Z,8Z,10R,11S,12S,14Z)-10-hydroxy-11,12-epoxyicosa-5,8,14-trienoate and (5Z,8R,9E,11S,12S,14Z)-8-hydroxy-11,12-epoxyicosa-5,9,14-trienoate [1]. The mouse enzyme has highest activity with (8S)-HPETE, producing (5Z,8S,9S,10R,11Z,14Z)-10-hydroxy-8,9-epoxyicosa-5,11,14-trienoate [2]. The enzymes also have the activity of EC 4.2.1.152, hydroperoxy icosatetraenoate dehydratase.
linoleic, arachidonic, and eicosapentaenoic acids, the methyl esters of arachidonic acid and linoleic acid, arachidonyl phosphatidylcholine, anandamide, and the cholesteryl ester of arachidonic acid are no substrates
intra-amniotic delivery of epidermal lipoxygenase-3 (eLOX-3) to mice at gestational day 14.5, both via an adenoviral vector and as recombinant protein, results in fetal growth restriction and intrauterine death. Periodic acid-Schiff staining and RT-PCR analysis of placentae from fetuses exposed to eLOX-3 indicate a lack of glycogen trophoblasts in the junctional zone. Placenta-specific gene expression is altered. Thus, the observed prenatal toxicity of eLOX-3 can be due to a strong effect on placental development. Placentae from eLOX-3-exposed fetuses have an altered structure. Short life-span of eLOX-3 null mice
metabolites of the epidermal lipoxygenase-3 (eLOX-3) are involved in various metabolic pathways. Intra-amniotic delivery of eLOX-3 to mice at gestational day 14.5, both via an adenoviral vector and as recombinant protein, results in fetal growth restriction and intrauterine death. Periodic acid-Schiff staining and RT-PCR analysis of placentae from fetuses exposed to eLOX-3 indicate a lack of glycogen trophoblasts in the junctional zone. Placenta-specific gene expression is altered. Thus, the observed prenatal toxicity of eLOX-3 can be due to a strong effect on placental development. eLOX-3 appears to have some impact on the expression of genes that indirectly influence fetal development
intra-amniotic delivery of eLOX-3 to mice at gestational day 14.5, both via an adenoviral vector and as recombinant protein, expression of the placenta-specific genes Tpbpa, Pcdh12, Muc1, Gcm1, SynA, and PL-1, encoding trophoblast-specific protein alpha, protocadherin 12, mucin 1, glial cell missing homologue 1, syncytin A, and placental lactogen 1, respectively, is analyzed in SYBR green-based real-time RT-PCR assays. Analysis of toxicity of eLOX-3 and dose-dependence of fetal survival, overview. Placentae from eLOX-3-exposed fetuses have an altered structure