Information on EC 5.4.4.4 - geraniol isomerase

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The expected taxonomic range for this enzyme is: Castellaniella defragrans

EC NUMBER
COMMENTARY hide
5.4.4.4
-
RECOMMENDED NAME
GeneOntology No.
geraniol isomerase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geraniol = (3S)-linalool
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta; myrcene degradation
-
-
Biosynthesis of secondary metabolites
-
-
Monoterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geraniol hydroxymutase
In absence of oxygen the bifunctional linalool dehydratase-isomerase can catalyse in vitro two reactions, the isomerization of (3S)-linalool to geraniol and the hydration of myrcene to (3S)-linalool, the latter activity being classified as EC 4.2.1.127, linalool dehydratase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 65Phen
UniProt
Manually annotated by BRENDA team
strain 65Phen
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geraniol
linalool
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geraniol
linalool
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
contains no prosthetic group
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
O2
the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool
Ti(III)citrate
1 mM, complete inhibition
additional information
not inhibited by 10% (v/v) DMSO. EDTA (5 mM) does not affect the enzyme activity
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
the aerobically purified enzyme is anaerobically activated in the presence of 2 mM dithiothreitol
S-adenosyl-L-methionine
40 mM, 2fold activation
additional information
the enzyme requires dithiothreitol as a reducing agent and an oxygen free microenvironment (less than 1% (v/v)) for the dehydration of linalool
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
geraniol
pH 9.0, 35°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
4 * 40000, SDS-PAGE
160000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli