Information on EC 5.4.3.9 - glutamate 2,3-aminomutase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.4.3.9
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RECOMMENDED NAME
GeneOntology No.
glutamate 2,3-aminomutase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-glutamate = 3-aminopentanedioate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
L-glutamate 2,3-aminomutase
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. It contains pyridoxal phosphate and a [4Fe-4S] cluster, which is coordinated by 3 cysteines and binds an exchangeable S-adenosyl-L-methionine molecule. During the reaction cycle, the AdoMet forms a 5'-deoxyadenosyl radical, which is regenerated at the end of the reaction.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene eam
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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glutamate 2,3-aminomutase is a member of the radical SAM superfamily of enzymes. The highly active glutamate 2,3-aminomutase that is homologous with lysine 2,3-aminomutase
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-glutamate
3-aminopentanedioate
show the reaction diagram
L-glutamate
3-aminopentanedioate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-glutamate
3-aminopentanedioate
show the reaction diagram
L-glutamate
3-aminopentanedioate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
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required, iron-sulfide cluster [4Fe-4S], the iron-sulfur cluster in glutamate 2,3-aminomutase is labile duirng purification
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
O2
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glutamate-specific 2,3-aminomutases is sensitive to molecular oxygen which oxidizes the [4Fe-4S]1+ cluster required for catalysis to [4Fe–4S]2+
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli by ammonium sulfate fractuionation, hydrophobic interaction chromatography, anion exchange chromatography, another step of ammonium sulfate fractionation, centrifugation, and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene eam, DNA and amio acid sequence determination and analysis, functional expression in Escherichia coli BL21 (DE3) CodonPlus RILP cells
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