Information on EC 5.4.2.3 - phosphoacetylglucosamine mutase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
5.4.2.3
-
RECOMMENDED NAME
GeneOntology No.
phosphoacetylglucosamine mutase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
group transfer
-
-
intramolecular, phosphate group
-
isomerization
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
-
-
Biosynthesis of antibiotics
-
-
chitin derivatives degradation
-
-
Metabolic pathways
-
-
UDP-N-acetyl-D-galactosamine biosynthesis II
-
-
UDP-N-acetyl-D-glucosamine biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
N-Acetyl-alpha-D-glucosamine 1,6-phosphomutase
The enzyme is activated by N-acetyl-alpha-D-glucosamine 1,6-bisphosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9027-51-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Coffea sp.
coffee plant
-
-
Manually annotated by BRENDA team
gene nst
UniProt
Manually annotated by BRENDA team
strain MR4
-
-
Manually annotated by BRENDA team
strain MR4
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene PAGM
UniProt
Manually annotated by BRENDA team
gene PAGM
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
loss-of-function mutations in the nst gene, which encodes PGM3, block fibroblast growth factor signaling during embryonic mesoderm and tracheal development. In nst mutants with an 80% reduction in the amount of UDP-GlcNAc, O-GlcNAcylation is compromised. Embryos maternally and zygotically mutant for nst exhibit defects in mesoderm layer formation during gastrulation that are similar to those observed in embryos with mutations in the gene encoding the fibroblast growth factor receptor Heartless (Htl). Zygotic nst mutants (nstZ) exhibit normal dorsal mesoderm differentiation, embryos homozygous for the hypomorphic htlYY262 allele show a reduction in dorsal mesoderm. Phenotypes, overview. Dof is the critical component in MAPK activation affected in nst mutants
metabolism
Nst acts downstream of fibroblast growth factor receptor signaling
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucose-1-phosphate
D-glucose-6-phosphate
show the reaction diagram
-
-
-
?
Glucose 1-phosphate
Glucose 6-phosphate
show the reaction diagram
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
show the reaction diagram
N-Acetyl-D-glucosamine 1-phosphate
N-Acetyl-D-glucosamine 6-phosphate
show the reaction diagram
N-acetyl-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
N-Acetylglucosamine 6-phosphate
?
show the reaction diagram
N-Acetylglucosamine 6-phosphate
N-Acetylglucosamine 1-phosphate
show the reaction diagram
N-acetylglucosamine-1-phosphate
N-acetylglucosamine-6-phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-alpha-D-glucosamine 1-phosphate
N-acetyl-D-glucosamine 6-phosphate
show the reaction diagram
N-acetyl-D-glucosamine 6-phosphate
N-acetyl-alpha-D-glucosamine 1-phosphate
show the reaction diagram
Q9VTZ4
-
-
-
r
N-Acetylglucosamine 6-phosphate
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
divalent metal ion required. Mn2+ is less efficient than Mg2+
phosphate
-
covalently linked to the enzyme. The phosphate-protein linkage is stable in mild acid, at 50 C for up to 2 h. However phosphate is completely released under mild alkaline conditions at 50 C within 1 h
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Be2+
-
0.02 mM in presence of 1 mM Mg2+, complete inhibition
Cd2+
-
0.5 mM in presence of 1 mM Mg2+, complete inhibition
Cu2+
-
0.5 mM in presence of 1 mM Mg2+, complete inhibition
D-glucose 1-phosphate
competitive inhibition
D-glucose 6-phosphate
competitive inhibition
Mn2+
-
1 mM in presence of 1 mM Mg2+, complete inhibition
Zn2+
-
0.5 mM in presence of 1 mM Mg2+, complete inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-hydroxyquinoline
-
metal chelator required
Cys
-
metal chelator required
diethyldithiocarbamate
-
metal chelator required
diethyldithiocarbamide
-
or histidine required for maximal activity
-
fructose 1,6-diphosphate
-
or glucose 1,6-diphosphate activates, Km: 0.018 mM
glucose 1,6-diphosphate
N-Acetylglucosamine 1,6-diphosphate
-
required for reaction with N-acetylglucosamine 1-phosphate
sulfhydryl compound
-
increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54
D-glucose-1-phosphate
pH 7.4, 26C
0.39 - 0.658
glucose 1-phosphate
0.046
N-acetyl-alpha-D-glucosamine
-
0.014
N-acetyl-D-glucosamine 6-phosphate
pH 7.3, 37C
0.0237 - 0.7
N-acetylglucosamine 1-phosphate
3.6
N-acetylglucosamine 6-phosphate
-
-
0.046
N-acetylglucosamine-1-phosphate
pH 7.4, 26C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.79 - 2.94
N-acetyl-alpha-D-glucosamine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16
N-acetyl-D-glucosamine 6-phosphate
Trypanosoma brucei
F4NCC3
pH 7.3, 37C
1092
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0063
D-glucose 1-phosphate
Trypanosoma brucei
F4NCC3
versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37C
0.0083
D-glucose 6-phosphate
Trypanosoma brucei
F4NCC3
versus N-acetyl-D-glucosamine 6-phosphate, pH 7.3, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.056
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
Tris-HCl buffer
7 - 7.5
-
Tris-maleate buffer
7.2 - 9.1
broad optimum
7.3
-
Tris-acetate buffer
7.9
-
formation of N-acetylglucosamine 1-phosphate
8.2
-
Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9
-
pH 6.5: about 45% of maximal activity, pH 9.0: about 40% of maximal activity, Tris-maleate buffer
7 - 8.5
-
about 60% of maximal activity at pH 7.0 and at pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
Coffea sp.
-
-
Manually annotated by BRENDA team
-
highest activity of reticulo-endothelial tissues tested
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59000
-
gel filtration
60000
-
gel filtration
60600
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
both in apo form and in the complex forms with substrate and product. Protein consists of four domains. of which three domains have essentially the same fold. The catalytic cleft is formed by four loops from each domains. The N-acetyl group of the substrate is recognized by V370 and N389 in domain 3, from which the substrate specificity arises. Substrate rotates about 180 on the axis linking C4 and the midpoint of the C5-O5 bond in the reaction
-
space group P21, crystal diffract to beyond 1.8 A
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme is present in two forms of different heat stability
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme loses more than 90% activity upon one freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-16C, stable for several months
-
0-4C, pH 6.5, stable for 7 days
-
0C, 30% sucrose or 30% glycerol, 50% loss of activity after 3 weeks. Addition of albumin or varying the pH does not appreciably enhance the stability of the enzyme
-
frozen enzyme, pH 6.5, stable for at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glutathione affinity column
recombinant CaAgm1-GST fusion protein
-
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant HsAgm1-GST fusion protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression as glutathione S-transferase fusion protein
-
expression in Escherichia coli
gene nst, DNA and amino acid sequence determination, gene nst is allelic to CG10627, expression analysis
gene PAGM, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D276A
no activity
D276E
extremely low activity
D278A
no activity
D278E
no activity
D280A
extremely low activity
D70A
65% of wild type activity
G277A
79% of wild type activity
G499A
0.5% of wild type activity
G58A
20% of wild type activity
G72A
3% of wild type activity
H65A
no activity
H65R
very low activity
K74A
no activity
N66A
1.3% of wild type activity
P67A
100% of wild type activity
R281A
no activity
R281K
no activity
R496A
extremely low activity
R498A
8% of wild type activity
S64A
no activity
S64T
no activity
T500A
extremely low activity
T62A
106% of wild type activity
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
enzymic synthesis of beta-UDP-N-acetylglucosamine using UTP:N-acetylglucosamine 1-phosphate phosphotransferase and N-acetyl-D-glucosamine 1,6-phosphomutase
Show AA Sequence (112 entries)
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