Information on EC 5.3.99.10 - thiazole tautomerase

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The expected taxonomic range for this enzyme is: Bacillus subtilis

EC NUMBER
COMMENTARY hide
5.3.99.10
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RECOMMENDED NAME
GeneOntology No.
thiazole tautomerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate = 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Thiamine metabolism
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thiazole biosynthesis II (aerobic bacteria)
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SYSTEMATIC NAME
IUBMB Comments
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate isomerase
The enzyme catalyses the irreversible aromatization of the thiazole moiety of 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene tenI
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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TenI is involved in the thiamin thiazole biosynthesis. In Bacillus subtilis, the thiazole tautomer (R,Z)-2-(2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate is formed by an oxidative condensation of glycine, 1-deoxy-D-xylulose-5-phosphate, and cysteine
physiological function
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TenI shows no thiamin phosphate synthase activity, but functionsas a thiazole tautomerase, substrates phosphate and histidine 122 act as the acid/base residues involved in catalysis, reaction mechanism, overview. TenI does not affect glycine oxidase, thiamin phosphate synthase, or ThiS-COSH formation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate
2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate
show the reaction diagram
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ir
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
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assay at
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme TenI complexed with its reaction product cThz-P bound in the active site, hanging drop vapor diffusion method, liand-free TenI is grown from 1.65 to 1.75 M ammonium sulfate, 100 mM bicine, pH 8.7, 9.6, 2% PEG 400 w/v, and 8 mM L-cysteine, crystals are first dialyzed into 2.38 M sodium malonate, pH 7.0, to remove the sulfate ions by gradually increasing the sodium malonate concentration and decreasing the ammonium sulfate concentration, and soaked overnight in 2.38 M sodium malonate, 21.5 mM bicine, pH 9.0, 0.01% PEG400 w/v, 0.5 mM L-cysteine, 4% glycerol, and 11.5 mM cThz-P, X-ray diffraction structure determination and analysis
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged TenI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene tenI, overexpression as His-tagged enzyme in Escherichia coli strain BL21(DE3)
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