Information on EC 5.3.3.B2 - linoleate (10E,12Z)-isomerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.3.3.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
linoleate (10E,12Z)-isomerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate
show the reaction diagram
linoleic acid = (10E,12Z)-octadeca-10,12-dienoic acid
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
linoleate DELTA9-cis-DELTA10-trans-isomerase
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(6Z,9Z,12Z)-octadecatrienoic acid
(6Z,10E,12Z)-octadecatrienoic acid
show the reaction diagram
(9Z,12Z)-octadecadienoic acid
(10E,12Z)-octadecadienoic acid
show the reaction diagram
9-cis,12-cis-linoleic acid
10-trans,12-cis-linoleic acid
show the reaction diagram
linoleic acid
(10E,12Z)-octadeca-10,12-dienoic acid
show the reaction diagram
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-
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9-cis,12-cis-linoleic acid
10-trans,12-cis-linoleic acid
show the reaction diagram
linoleic acid
(10E,12Z)-octadeca-10,12-dienoic acid
show the reaction diagram
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-
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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flavin is a putative cofactor, either FAD or FMN
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
linoleic acid
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49077
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x * 49077, sequence calculaion
50000
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x * 50000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
six crystal structures in apo and product-bound forms, by single isomorphous replacement with anomalous scattering, SIRAS
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
of the recombinant protein by glutathione sepharose chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, functional overexpression in Lactococcus lactis strain NZ9800 and Escherichia coli strain DH5alpha, which produce 10-trans,12-cis-linoleic acid, overview
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expressed in Yarrowia lipolytica
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expression in Escherichia coli, in Saccharomyces cerevisiae and in transgenic tabacco cells
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expression in transgenic Oryza sativa plants by Agrobacterium tumefaciens-mediated transformation under the control of seed-specific promoters from the oleosin and globulin genes, analysis of oil composition in transgenic seeds, overview
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overexpression in Escherichia coli as a GST-fusion protein
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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the enzyme shows the ability to produce fatty acid components of vegetable oils with novel physiological activities in crops
nutrition
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the enzyme shows the ability to produce fatty acid components of vegetable oils with novel physiological activities in crops