Information on EC 5.3.3.9 - prostaglandin-A1 DELTA-isomerase

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The expected taxonomic range for this enzyme is: Eutheria

EC NUMBER
COMMENTARY
5.3.3.9
-
RECOMMENDED NAME
GeneOntology No.
prostaglandin-A1 DELTA-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
(13E)-(15S)-15-hydroxy-9-oxoprosta-10,13-dienoate = (13E)-(15S)-15-hydroxy-9-oxoprosta-11,13-dienoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(13E)-(15S)-15-hydroxy-9-oxoprosta-10,13-dienoate DELTA10-DELTA11-isomerase
Interconverts prostaglandin A1 and prostaglandin C1.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Isomerase, prostaglandin A1DELTA-
-
-
-
-
PGA isomerase
-
-
-
-
Prostaglandin A isomerase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9055-01-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
weak activity
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
no activity in Bos taurus
fetal calf
-
-
Manually annotated by BRENDA team
no activity in Bos taurus
ox
-
-
Manually annotated by BRENDA team
no activity in Gallus gallus
-
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
-
-
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
the product is prostaglandin C1, which is unstable under mild alkaline conditions and isomerizes to prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
i.e. prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
i.e. prostaglandin B1
-
(13E)-(15S)-15-Hydroxy-9-oxoprosta-10,13-dien-1-oic acid
(13E)-(15S)-15-Hydroxy-9-oxoprosta-8[12]13-dien-1-oic acid
show the reaction diagram
-
i.e. prostaglandin A1
i.e. prostaglandin B1
-
(5Z,13E)-(15S)-15-Hydroxy-9-oxoprosta-5,10,13-trien-1-oic acid
?
show the reaction diagram
-
i.e. prostaglandin A2
-
-
-
(5Z,13E)-(15S)-15-Hydroxy-9-oxoprosta-5,10,13-trien-1-oic acid
?
show the reaction diagram
-
i.e. prostaglandin A2
-
-
-
15-Epiprostaglandin A2
?
show the reaction diagram
-
-
-
-
-
15-Epiprostaglandin A2
?
show the reaction diagram
-
-
-
-
-
19-Hydroxyprostaglandin A1
?
show the reaction diagram
-
-
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
13,14-Dihydroprostaglandin A1
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
no effect at 1 mM
5,5'-dithiobis(2-nitrobenzoate)
-
-
NEM
-
1 mM, 90% inhibition
p-mercuribenzoate
-
-
Urea
-
8 M, completely and irreversibly inactivates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.025
Prostaglandin A1
-
-
0.04
Prostaglandin A1
-
in absence of rabbit albumin
0.05
Prostaglandin A1
-
in presence of rabbit albumin
0.054
Prostaglandin A1
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
8.5
-
-
9.5 - 10
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 10
-
pH 6.0: about 30% of maximal activity, pH 10.0: about 35% of maximal activity
6 - 9
-
below pH 6 and above pH 9, activity rapidly declines
6.5 - 10
-
pH 6.5: about 35% of maximal activity, pH 9.5-10.0: maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 75
-
about 30% of maximal activity at 30C and 75C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
28000
-
-
3031
110000
-
gel filtration
3033
115000
-
gel filtration
3030
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2
-
4C, 15 h, enzyme activity in serum is stable
3028
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
-
20 min, enzyme activity in plasma is stable
3027
50
-
10 min, denaturation commences above 50C
3029
55
-
pH 7, 10 min, no denaturation
3030
60
-
3 h, enzyme activity in plasma is stable
3028
60
-
10 min, rather stable at
3031
62
-
10 min, complete inactivation
3029
65
-
20 min, inactivation
3033
70
-
20 min, complete loss of enzyme activity in plasma
3027
70
-
20 min, enzyme activity in serum is stable
3028
70
-
-
3028
70
-
20 min, enzyme activity in serum is stable
3028
70
-
-
3030
70
-
10 min, 90% loss of activity
3030
70
-
-
3030
70
-
10 min, 50% loss of activity
3031
70
-
-
3032
70
-
20 min, 93% loss of activity
3032
70
-
20 min, 50-75% loss of activity
3032
70
-
-
3032
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
10% loss of activity after dialysis
-
loss of activity after pepsin digestion
-
10% loss of activity after dialysis
-
8 M urea completely and irreversibly inactivates
-
loss of activity after pepsin digestion
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-