Information on EC 5.3.3.7 - aconitate DELTA-isomerase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
5.3.3.7
-
RECOMMENDED NAME
GeneOntology No.
aconitate DELTA-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
trans-aconitate = cis-aconitate
show the reaction diagram
-
-
-
-
trans-aconitate = cis-aconitate
show the reaction diagram
a single catalytic base is found on the enzyme surface, the mechanism involves deprotonation to form a carbanion
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
aconitate DELTA2-DELTA3-isomerase
cis-Aconitate is used to designate the isomer (Z)-prop-1-ene-1,2,3-tricarboxylate. This isomerization could take place either in a direct cis-trans interconversion or by an allylic rearrangement; the enzyme has been shown to catalyse the latter change.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Aconitate isomerase
-
-
-
-
AI
-
-
-
-
Isomerase, aconitate DELTA-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37318-48-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 1474
-
-
Manually annotated by BRENDA team
Alcaligenes faecalis 1474
strain 1474
-
-
Manually annotated by BRENDA team
var. botrytis, weak activity
-
-
Manually annotated by BRENDA team
strain 8810
-
-
Manually annotated by BRENDA team
Burkholderia gladioli 8810
strain 8810
-
-
Manually annotated by BRENDA team
strain 1391
-
-
Manually annotated by BRENDA team
Clostridium butyricum 1391
strain 1391
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
strain Mul1 (Mulberry)
-
-
Manually annotated by BRENDA team
Enterobacter cloacae Mul1
strain Mul1 (Mulberry)
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
no activity in Apium graveolens
leaf, root
-
-
Manually annotated by BRENDA team
no activity in Araucaria excelsa
root
-
-
Manually annotated by BRENDA team
no activity in Daucus carota
leaf, root
-
-
Manually annotated by BRENDA team
no activity in Elaeis oleifera
leaf blade
-
-
Manually annotated by BRENDA team
no activity in Malus domestica
leaf, fruit
-
-
Manually annotated by BRENDA team
pv. milletiae strain 301749; strain DW1 (Mulberry); strain Mei3 (Mulberry pyralid)
-
-
Manually annotated by BRENDA team
Pantoea agglomerans DW1
strain DW1 (Mulberry)
-
-
Manually annotated by BRENDA team
Pantoea agglomerans Mei3
strain Mei3 (Mulberry pyralid)
-
-
Manually annotated by BRENDA team
strain 301720, 301718 and 101722 from rice; strain Mei (Mulberry pyralid); strain TM2 (Mulberry)
-
-
Manually annotated by BRENDA team
Pantoea ananatis 301720
strain 301720, 301718 and 101722 from rice
-
-
Manually annotated by BRENDA team
Pantoea ananatis Mei
strain Mei (Mulberry pyralid)
-
-
Manually annotated by BRENDA team
Pantoea ananatis TM2
strain TM2 (Mulberry)
-
-
Manually annotated by BRENDA team
strain 13525; strain LRB3W1
-
-
Manually annotated by BRENDA team
Pseudomonas fluorescens 13525
strain 13525
-
-
Manually annotated by BRENDA team
Pseudomonas fluorescens LRB3W1
strain LRB3W1
-
-
Manually annotated by BRENDA team
strain Shizu
-
-
Manually annotated by BRENDA team
Pseudomonas marginalis Shizu
strain Shizu
-
-
Manually annotated by BRENDA team
strain A 3.12
-
-
Manually annotated by BRENDA team
Pseudomonas putida A 3.12
strain A 3.12
-
-
Manually annotated by BRENDA team
pv. atropurpurea; pv. broussnetiae; pv. coronafaciens; pv. glycinea; pv. lachrymans; pv. maculicola H330, not pv. maculicola H1 and H3-9; pv. mori; pv. morsprunorum; pv. phaseolicola
-
-
Manually annotated by BRENDA team
strain Ko8 (Brassica campestris); strain M328 and M303
-
-
Manually annotated by BRENDA team
Pseudomonas viridiflava Ko8
strain Ko8 (Brassica campestris)
-
-
Manually annotated by BRENDA team
Pseudomonas viridiflava M328
strain M328 and M303
-
-
Manually annotated by BRENDA team
strain F-1-1 (Salvia splendens)
-
-
Manually annotated by BRENDA team
Serratia marcescens F-1-1
strain F-1-1 (Salvia splendens)
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
-
r
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
Pseudomonas putida A 3.12
-
-
-
-
trans-Aconitate
cis-Aconitate
show the reaction diagram
Pseudomonas marginalis Shizu, Pantoea agglomerans DW1, Pantoea ananatis 301720, Pseudomonas viridiflava M328, Pseudomonas fluorescens 13525, Pantoea ananatis TM2, Enterobacter cloacae Mul1, Burkholderia gladioli 8810, Pantoea ananatis Mei, Pseudomonas viridiflava Ko8, Pseudomonas fluorescens LRB3W1, Alcaligenes faecalis 1474, Clostridium butyricum 1391, Serratia marcescens F-1-1, Pantoea agglomerans Mei3
-
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
glycerol
-
increases activity
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.18
7.7
cis-aconitate
-
Km-value is pH-dependent and increases from 0.18 mM at pH 7.15 to 7.7 mM at pH 9.25
1.05
1.9
cis-aconitate
-
in presence of 20% glycerol, over a 4fold range in enzyme concentration, the Km-value is inversely dependent on the enzyme concentration
9
25
cis-aconitate
-
in absence of glycerol, over a 4fold range in enzyme concentration, the Km-value is inversely dependent on the enzyme concentration
3.3
-
trans-aconitate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
9.3
-
pH 7.5: about 30% of maximal activity, pH 9.3: about 80% loss of activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
of germinating seeds
Manually annotated by BRENDA team
Pseudomonas fluorescens 13525, Pseudomonas fluorescens LRB3W1, Pseudomonas putida A 3.12
-
-
-
Manually annotated by BRENDA team
-
weak activity
Manually annotated by BRENDA team
-
weak activity
Manually annotated by BRENDA team
-
of germinating seeds
Manually annotated by BRENDA team
-
immature
Manually annotated by BRENDA team
additional information
-
no activity in immature inflorescence
Manually annotated by BRENDA team
additional information
-
no activity in fruit
Manually annotated by BRENDA team
additional information
-
no activity in bulb
Manually annotated by BRENDA team
additional information
-
no activity in mature seed
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
almost 90% of the activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
78000
-
-
gel filtration
80000
-
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
method for estimation of enzyme activity in plant material
analysis
-
a simple sensitive and specific method for measuring aconitate isomerase in plants, which depends on the release of tritium from labeled trans-aconitate
analysis
-
method for estimation of enzyme activity in plant material
analysis
-
a simple sensitive and specific method for measuring aconitate isomerase in plants, which depends on the release of tritium from labeled trans-aconitate
analysis
-
method for estimation of enzyme activity in plant material