Information on EC 5.3.3.7 - aconitate DELTA-isomerase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
5.3.3.7
-
RECOMMENDED NAME
GeneOntology No.
aconitate DELTA-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
trans-aconitate = cis-aconitate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
C5-Branched dibasic acid metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
aconitate DELTA2-DELTA3-isomerase
cis-Aconitate is used to designate the isomer (Z)-prop-1-ene-1,2,3-tricarboxylate. This isomerization could take place either in a direct cis-trans interconversion or by an allylic rearrangement; the enzyme has been shown to catalyse the latter change.
CAS REGISTRY NUMBER
COMMENTARY hide
37318-48-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
mulberry
-
-
Manually annotated by BRENDA team
mulberry
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
weak activity
-
-
Manually annotated by BRENDA team
no activity in Apium graveolens
leaf, root
-
-
Manually annotated by BRENDA team
no activity in Araucaria excelsa
root
-
-
Manually annotated by BRENDA team
no activity in Daucus carota
leaf, root
-
-
Manually annotated by BRENDA team
no activity in Elaeis oleifera
leaf blade
-
-
Manually annotated by BRENDA team
no activity in Malus domestica
leaf, fruit
-
-
Manually annotated by BRENDA team
; mulberry; pv. milletiae strain 301749
-
-
Manually annotated by BRENDA team
mulberry
-
-
Manually annotated by BRENDA team
and strains 301720, 301718 and 101722 isolated from rice; mulberry; Mulberry pyralid
-
-
Manually annotated by BRENDA team
and strains 301720, 301718 and 101722 isolated from rice
-
-
Manually annotated by BRENDA team
Mulberry pyralid
-
-
Manually annotated by BRENDA team
mulberry
-
-
Manually annotated by BRENDA team
pv. atropurpurea; pv. broussnetiae; pv. coronafaciens; pv. glycinea; pv. lachrymans; pv. maculicola H330, not pv. maculicola H1 and H3-9; pv. mori; pv. morsprunorum; pv. phaseolicola
-
-
Manually annotated by BRENDA team
and strain M303; Brassica campestris
-
-
Manually annotated by BRENDA team
and strain M303
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isolated from Salvia splendens
-
-
Manually annotated by BRENDA team
isolated from Salvia splendens
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans-Aconitate
cis-Aconitate
show the reaction diagram
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerol
-
increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18 - 25
cis-aconitate
3.3
trans-aconitate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 9.3
-
pH 7.5: about 30% of maximal activity, pH 9.3: about 80% loss of activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
of germinating seeds
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
almost 90% of the activity
Manually annotated by BRENDA team
-
low activity
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
78000
-
gel filtration
80000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis