Information on EC 5.3.3.17 - trans-2,3-dihydro-3-hydroxyanthranilate isomerase

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The expected taxonomic range for this enzyme is: Pseudomonas fluorescens

EC NUMBER
COMMENTARY hide
5.3.3.17
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RECOMMENDED NAME
GeneOntology No.
trans-2,3-dihydro-3-hydroxyanthranilate isomerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate = (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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Phenazine biosynthesis
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phenazine-1-carboxylate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate isomerase
The enzyme is involved in phenazine biosynthesis. The product probably spontaneously dimerises to 1,4,5a,6,9,10a-hexahydrophenazine-1,6-dicarboxylate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 2-79
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate
(1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(5S,6S)-6-amino-5-hydroxycyclohexane-1,3-diene-1-carboxyate
(1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate
show the reaction diagram
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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two protein species with MW 32048 Da and 32224 Da, MALDI-TOF mass-spectrometry
32218
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2 * 32218, histidine-tagged enzyme
42000
gel filtration
66070
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laser light scattering in solution
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1.8 A crystal structure of PhzF, hanging drop vapor diffusion method
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hanging-drop vapor-diffusion method. Crystals of unliganded PhzF belong to space group P3(2)21 with a a = b = 56 A, and c = 156 A and hold one monomer per asymmetric unit. Complexes of PhzF (with 3-hydroxyanthranilic acid and trans-2,3-dihydro-3-hydroxyanthranilic acid) crystallize in space group P2(1)2(1)2 with cell parameters of a = 93 A, b = 100 A, and c = 57 A and contain one dimer in the asymmetric unit
hanging-drop vapour-diffusion method. Crystallized from PEG 4000/ammonium sulfate/sodium citrate pH 5.6. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 56.3, c = 156.4 A. They contain one monomer in the asymmetric unit and diffract to better than 1.7 A on synchrotron beamlines. Crystals of seleno-L-methionine-labelled PhzF have been obtained
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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large-scale expression in Escherichia coli
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Show AA Sequence (194 entries)
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