Information on EC 5.3.2.8 - 4-oxalomesaconate tautomerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.3.2.8
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RECOMMENDED NAME
GeneOntology No.
4-oxalomesaconate tautomerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate = (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gallate degradation II
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syringate degradation
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methylgallate degradation
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gallate degradation I
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protocatechuate degradation I (meta-cleavage pathway)
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Benzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
4-oxalomesaconate keto-enol-isomerase
This enzyme has been characterized from the bacterium Pseudomonas putida KT2440 and is involved in the degradation pathway of syringate and 3,4,5-trihydroxybenzoate. It catalyses the interconversion of two of the tautomers of 4-oxalomesaconate, a reaction that can also occur spontaneously.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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disruption of the galD gene does not prevent growth of the Pseudomonas putida KTGALdgalD mutant strain in 5 mM gallic acid, but the mutant strain grows poorly at gallic acid concentrations lower than 2 mM
physiological function
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a functional galD gene is important for an efficient gallic acid degradation
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
show the reaction diagram
4-chloronitrobenzene
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2E)-2-(2-methylprop-2-en-1-ylidene)-4-oxopentanedioic acid
(2Z,4E)-2-hydroxy-4-(2-methylprop-2-en-1-ylidene)pent-2-enedioic acid
show the reaction diagram
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37600
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calculated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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