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Information on EC 5.3.2.7 - ascopyrone tautomerase and Organism(s) Phanerodontia chrysosporium and UniProt Accession P84193

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EC Tree
     5 Isomerases
         5.3 Intramolecular oxidoreductases
             5.3.2 Interconverting keto- and enol-groups
                5.3.2.7 ascopyrone tautomerase
IUBMB Comments
This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. The other enzymes involved in this pathway are EC 4.2.1.110 (aldos-2-ulose dehydratase), EC 4.2.1.111 (1,5-anhydro-D-fructose dehydratase) and EC 4.2.2.13 [exo-(1->4)-alpha-D-glucan lyase]. Ascopyrone P is an anti-oxidant .
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Phanerodontia chrysosporium
UNIPROT: P84193
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The taxonomic range for the selected organisms is: Phanerodontia chrysosporium
The enzyme appears in selected viruses and cellular organisms
Synonyms
ascopyrone tautomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AUDH
bifunctional enzyme with EC 4.2.1.110 D-arabino-hex-2-ulose dehydratase and ascopyrone M isomerase activities
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose DELTA3-DELTA1-isomerase
This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. The other enzymes involved in this pathway are EC 4.2.1.110 (aldos-2-ulose dehydratase), EC 4.2.1.111 (1,5-anhydro-D-fructose dehydratase) and EC 4.2.2.13 [exo-(1->4)-alpha-D-glucan lyase]. Ascopyrone P is an anti-oxidant [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ascopyrone M
microthecin
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ascopyrone M
microthecin
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
for microthecin formation
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 5.3
isoelectric focusing
5.5
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cf. EC 4.2.1.110
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AUD_PHACH
900
0
98746
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
98700
2 * 98700, SDS-PAGE
98746
2 * 98746, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.0), 0.2 M MgCl2 and 16% (w/v) polyethylene glycol 6000
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Hansenula polymorpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Claesson, M.; Lindqvist, Y.; Madrid, S.; Sandalova, T.; Fiskesund, R.; Yu, S.; Schneider, G.
Crystal structure of bifunctional aldos-2-ulose dehydratase/isomerase from Phanerochaete chrysosporium with the reaction intermediate ascopyrone M
J. Mol. Biol.
417
279-293
2012
Phanerodontia chrysosporium (P84193)
Manually annotated by BRENDA team