Information on EC 5.3.2.7 - ascopyrone tautomerase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Phanerochaete chrysosporium

EC NUMBER
COMMENTARY hide
5.3.2.7
-
RECOMMENDED NAME
GeneOntology No.
ascopyrone tautomerase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose = 1,5-anhydro-4-deoxy-D-glycero-hex-1-en-3-ulose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycogen degradation III (via anhydrofructose)
-
-
SYSTEMATIC NAME
IUBMB Comments
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose DELTA3-DELTA1-isomerase
This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. The other enzymes involved in this pathway are EC 4.2.1.110 (aldos-2-ulose dehydratase), EC 4.2.1.111 (1,5-anhydro-D-fructose dehydratase) and EC 4.2.2.13 [exo-(1->4)-alpha-D-glucan lyase]. Ascopyrone P is an anti-oxidant [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Anthracobia melaloma
-
-
-
Manually annotated by BRENDA team
cf. EC 4.2.1.110
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose
1,5-anhydro-4-deoxy-D-glycero-hex-1-en-3-ulose
show the reaction diagram
ascopyrone M
microthecin
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,5-anhydro-4-deoxy-D-glycero-hex-3-en-2-ulose
1,5-anhydro-4-deoxy-D-glycero-hex-1-en-3-ulose
show the reaction diagram
ascopyrone M
microthecin
show the reaction diagram
P84193
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3873
Anthracobia melaloma
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
Anthracobia melaloma
-
optimal range: 5.0-6.0
7.5
for microthecin formation
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 5.3
isoelectric focusing
5.5
calculated from amino acid sequence
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
98700
2 * 98700, SDS-PAGE
98746
2 * 98746, calculated from amino acid sequence
140000
Anthracobia melaloma
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 98700, SDS-PAGE; 2 * 98746, calculated from amino acid sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.0), 0.2 M MgCl2 and 16% (w/v) polyethylene glycol 6000
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Hansenula polymorpha