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Information on EC 5.3.2.6 - 2-hydroxymuconate tautomerase and Organism(s) Pseudomonas sp. and UniProt Accession P49172

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IUBMB Comments
Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enol form (2Z,4E)-2-hydroxyhexa-2,4-dienedioate is produced as part of this pathway and is converted to the keto form (3E)-2-oxohex-3-enedioate by the enzyme . Another keto form, (4E)-2-oxohex-4-enedioate (4-oxalocrotonate), was originally thought to be produced by the enzyme [1,2] but later shown to be produced non-enzymically .
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Pseudomonas sp.
UNIPROT: P49172
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas sp.
The enzyme appears in selected viruses and cellular organisms
Synonyms
4-oxalocrotonate tautomerase, afdmpi, hpdmpi, sar1376, 2-hydroxymuconate tautomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-oxalocrotonate tautomerase
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4-oxalocrotonate tautomerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate = (3E)-2-oxohex-3-enedioate
show the reaction diagram
substrate specificity and one-base molecular mechanism, overview
SYSTEMATIC NAME
IUBMB Comments
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase
Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enol form (2Z,4E)-2-hydroxyhexa-2,4-dienedioate is produced as part of this pathway and is converted to the keto form (3E)-2-oxohex-3-enedioate by the enzyme [6]. Another keto form, (4E)-2-oxohex-4-enedioate (4-oxalocrotonate), was originally thought to be produced by the enzyme [1,2] but later shown to be produced non-enzymically [5].
CAS REGISTRY NUMBER
COMMENTARY hide
85876-28-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-Bromopyruvate
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a active-site-directed irreversible inhibitor, modification of three active sites per hexamer abolishes essentially all activity of the hexamer, pH dependence of inactivation
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.75
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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modification by 3-bromopyruvate of three active sites per hexamer abolishes essentially all activity of the hexamer, spectrocopic and sequence analysis of labeled peptides, overview
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
subunits are arranged with 32 symmetry
hexamer
-
-
additional information
monomeric and dimeric structure comparison of 4-oxalocrotonate tautomerase with monomeric 5-carboxymethyl-2-hydroxymuconate isomerase, overview. The region of greatest similarity between the two enzymes is a large pocket that is proposed to be the active site
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
4-OT from 80% saturated ammonium sulfate solution containing 100 mM HEPES, pH 7.5, and 1% PEG 200, 20°C, X-ray diffraction structure determination and analysis at 1.9-2.8 A resolution, heavy etal derivatization by soaking the crystals for 4 h in crystallization solution containing 2 mM K2PtCl4
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Subramanya, H.S.; Roper, D.I.; Dauter, Z.; Dodson, E.J.; Davies, G.J.; Wilson, K.S.; Wigley, D.B.
Enzymatic ketonization of 2-hydroxymuconate: specificity and mechanism investigated by the crystal structures of two isomerases
Biochemistry
35
792-802
1996
Pseudomonas sp. (P49172), Pseudomonas sp. CF 600 (P49172)
Manually annotated by BRENDA team
Stivers, J.; Abeygunawardana, C.; Mildvan, A.; Hajipour, G.; Whitman, C.; Chen, L.
Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies
Biochemistry
35
803-813
1996
Pseudomonas sp., Pseudomonas sp. CF 600
Manually annotated by BRENDA team