Information on EC 5.3.2.4 - TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose-forming)

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The expected taxonomic range for this enzyme is: Streptomyces fradiae

EC NUMBER
COMMENTARY hide
5.3.2.4
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RECOMMENDED NAME
GeneOntology No.
TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose-forming)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose = dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose biosynthesis
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dTDP-alpha-D-mycaminose biosynthesis
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Polyketide sugar unit biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose:dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose isomerase
The enzyme is involved in biosynthesis of D-mycaminose.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CDP-4-dehydro-6-deoxy-alpha-D-glucose
CDP-3-dehydro-6-deoxy-alpha-D-glucose
show the reaction diagram
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efficiency of conversion is low, requiring a high concentration of enzyme and a long incubation time to achieve significant turnover
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-
?
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose
dTDP-3-dehydro-2,6-dideoxy-alpha-D-glucose
show the reaction diagram
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efficiency is comparable to that with dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose
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-
?
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
dTDP-3-dehydro-6-deoxy-alpha-D-glucopyranose
show the reaction diagram
-
-
-
-
?
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-3-dehydro-6-deoxy-alpha-D-glucose
show the reaction diagram
-
the enzyme is involved in the D-mycaminose biosynthetic pathway
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
dTDP-3-dehydro-6-deoxy-alpha-D-glucose
show the reaction diagram
-
the enzyme is involved in the D-mycaminose biosynthetic pathway
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
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pH 7.5, 25C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025
CDP-4-dehydro-6-deoxy-alpha-D-glucose
Streptomyces fradiae
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pH 7.5, 25C
0.13
dTDP-4-dehydro-2,6-dideoxy-alpha-D-glucose
Streptomyces fradiae
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pH 7.5, 25C
0.1
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
Streptomyces fradiae
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pH 7.5, 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
Streptomyces fradiae
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pH 7.5, 25C
8916
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18806
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2 * 18806, calculated from sequence
19000
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2 * 19000, SDS-PAGE
31000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 18806, calculated from sequence; 2 * 19000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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