Information on EC 5.3.2.3 - TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose-forming)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.3.2.3
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RECOMMENDED NAME
GeneOntology No.
TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase (dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose-forming)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose = dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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dTDP-3-acetamido-alpha-D-fucose biosynthesis
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dTDP-D-ravidosamine and dTDP-4-acetyl-D-ravidosamine biosynthesis
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Polyketide sugar unit biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose:dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose isomerase
The enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. Four moieties of alpha-D-rhamnose and two moities of 3-acetamido-3,6-dideoxy-alpha-D-galactose form the repeating unit of the glycan chain in the S-layer of the bacterium Aneurinibacillus thermoaerophilus.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
?
show the reaction diagram
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no characterization of the product due to its low yield
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?
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
?
show the reaction diagram
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?
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose
dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19100
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2 * 19100, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallization of wild-type and selenomethionine-labeled FdtA, crystallization of the mutant proteins (H49N, H51N, H49N/H51N), hanging-drop method of vapor diffusion, crystallization trials are conducted at both 25C and 4C. Three-dimensional structure of this sugar isomerase complexed with dTDP and solved to 1.5 A resolution. Crystals belong to the space group P4(1)2(1)2 with unit cell dimensions of a = b = 62.7 A and c = 201.3 A and one dimer per asymmetric unit
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4C or -20C and supplemented with 50% glycerol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, the concentrated enzyme preparations are stable at 4C for several months with only a marginal decrease of enzyme activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H49N
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complete loss of activity
H49N/H51N
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complete loss of activity
H51N
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mutant retains some activity
H49N
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complete loss of activity
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H49N/H51N
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complete loss of activity
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H51N
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mutant retains some activity
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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synthesis of TDP-D-ravidosamine, necessary for in vitro glycosylation assays. TDP-D-ravidosamine is the anticipated sugar donor substrate of RavGT (the glycosyltransferase that links D-ravidosamine to the polyketide derived backbone defuco-gilvocarcin V). Defuco-gilvocarcin V exhibits superior anticancer/antibacterial activities