Information on EC 5.3.1.7 - mannose isomerase

Word Map on EC 5.3.1.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.3.1.7
-
RECOMMENDED NAME
GeneOntology No.
mannose isomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-Mannose = D-fructose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
D-mannose aldose-ketose-isomerase
Also acts on D-lyxose and D-rhamnose.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-25-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain S-48
-
-
Manually annotated by BRENDA team
strain S-48
-
-
Manually annotated by BRENDA team
strain M-1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
K12
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-fructose
D-mannose
show the reaction diagram
-
the conversion of 25% D-fructose is 29.4% after 2h
-
-
?
D-Lyxose
?
show the reaction diagram
D-Lyxose
D-Xylulose
show the reaction diagram
D-Mannose
?
show the reaction diagram
D-Mannose
D-Fructose
show the reaction diagram
D-Rhamnose
D-Rhamnulose
show the reaction diagram
-
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-fructose
D-mannose
show the reaction diagram
-
the conversion of 25% D-fructose is 29.4% after 2h
-
-
?
D-Mannose
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
-
2 mM, complete loss of activity
Co2+
-
2 mM, partial loss of activity
D-arabinose
-
-
D-mannitol
-
50 mM, slight inhibition
Fe2+
-
2 mM, complete loss of activity
Fe3+
-
2 mM, complete loss of activity
glucose
-
slight
glucuronic acid
-
-
GSH
-
5 mM
KCl
-
0.2 M
L-fucose
-
-
N-bromosuccinimide
-
partial inhibition at 0.1 mM, complete inhibition at 1 mM
NaCl
-
0.2 M
phenylmethylsulfonyl fluoride
-
partial inhibition at 0.1 mM, complete inhibition at 1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chelating agents
-
activation
sulfhydryl compounds
-
activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.1
D-fructose
-
-
300
D-Lyxose
-
-
1.4 - 80
D-mannose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
immobilized enzyme
7 - 7.2
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10.5
-
pH 6.0: about 65% of maximal activity, pH 10.5: about 50% of maximal activity
6 - 9.5
-
pH 6: about 35% of maximal activity, pH 9.5: about 40% of maximal activity
6.9 - 8.6
-
about 75% of maximal activity at pH 6.9 and 8.6
8 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 65
-
35°C: about 55% of maximal activity, 65°C: about 10% of maximal activity, 70°C: about 20% of maximal activity
40 - 68
-
40°C: about 45% of maximal activity, 68°C: about 25% of maximal activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
30% of the activity compared to mannose-grown cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
4 * 40000, SDS-PAGE
44000
-
2 * 44000, SDS-PAGE
90000
-
gel filtration
160000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
pH 7.5, 10 min, stable below
40
-
immobilized enzyme loses 46% of its activity after 4 d, soluble enzyme loses 45% of its activity after 4 d
50
-
pH 7.0, 10 min, stable
55
-
pH 7.5, 10 min, about 10% loss of activity
70
-
pH 7.5, 10 min, complete loss of activity
75
-
pH 7.0, 10 min, about 80% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no loss of activity after 24 h dialysis against 50 mM Tris-HCl buffer containing 1 mM EDTA
-
stable in presence of 1 mM 2-mercaptoethanol or EDTA
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
potential use of immobilized enzyme in a process for the production of fructose syrups containing a higher proportion of fructose