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Information on EC 5.3.1.6 - ribose-5-phosphate isomerase and Organism(s) Vibrio vulnificus and UniProt Accession Q7MHL9

for references in articles please use BRENDA:EC5.3.1.6
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IUBMB Comments
Also acts on D-ribose 5-diphosphate and D-ribose 5-triphosphate.
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This record set is specific for:
Vibrio vulnificus
UNIPROT: Q7MHL9
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Word Map
The taxonomic range for the selected organisms is: Vibrio vulnificus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
rpi, ribose-5-phosphate isomerase, phosphoriboisomerase, ribose phosphate isomerase, ctrpi, d-ribose-5-phosphate isomerase, ribosephosphate isomerase b, ribosephosphate isomerase a, ribose-5-phosphate isomerase b, ribose 5-phosphate isomerase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ribose 5-phosphate isomerase A
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5-Phosphoribose isomerase
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-
-
-
D-Ribose 5-phosphate isomerase
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-
-
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D-ribose-5-phosphate ketol-isomerase
-
-
-
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D-xylose ketol-isomerase
-
-
-
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Isomerase, ribose phosphate
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-
-
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Phosphopentoisomerase
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-
-
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Phosphopentose isomerase
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-
-
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Phosphoriboisomerase
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-
-
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Ribose phosphate isomerase
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-
-
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Ribose-5-P isomerase
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-
-
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Ribosephosphate isomerase A
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-
-
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Ribosephosphate isomerase B
-
-
-
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RPI
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
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intramolecular oxidoreduction
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-ribose-5-phosphate aldose-ketose-isomerase
Also acts on D-ribose 5-diphosphate and D-ribose 5-triphosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-83-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-ribose 5-phosphate
D-ribulose 5-phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-ribose 5-phosphate
D-ribulose 5-phosphate
show the reaction diagram
-
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
arabinose 5-phosphate
RpiA binds arabinose 5-phosphate located at the opening of the active site, the sugar ring of the inhibitor interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues, the phosphate group of arabinose 5-phosphate interacts with two water molecules, W51 and W82, binding mode of inhibitor, overview
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.77
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene rpiA
UniProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
recombinant detagged RpiA, dynamic light scattering and gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 20000-25000, RpiA
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
open form RpiA in complex with substrate ribose 5-phosphate, the closed form complexed with arabinose-5-phosphate, and the apo-RpiA, hanging drop vapor diffusion method, 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 150 mM KCl are mixed with 0.002 ml of a reservoir solution containing 50 mM succinate, pH 4.1, 180 mM ammonium sulfate, and 8% PEG 4000, 3 days, 20°C, for complexed enzyme addition of 20 mM ligand, X-ray diffraction structure determination and analysis at 1.49-2.07 A resolution, molecular replacement
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged RpiA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The His-tag is cleaved off and removed, folowed by anion exchange chromatography and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene rpiA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, T.G.; Kwon, T.H.; Min, K.; Dong, M.S.; Park, Y.I.; Ban, C.
Crystal structures of substrate and inhibitor complexes of ribose 5-phosphate isomerase A from Vibrio vulnificus YJ016
Mol. Cells
27
99-103
2009
Vibrio vulnificus (Q7MHL9), Vibrio vulnificus
Manually annotated by BRENDA team