Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 5.3.1.6 - ribose-5-phosphate isomerase

for references in articles please use BRENDA:EC5.3.1.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Also acts on D-ribose 5-diphosphate and D-ribose 5-triphosphate.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
UNIPROT: Q4CQE2
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
rpi, ribose-5-phosphate isomerase, phosphoriboisomerase, ribose phosphate isomerase, ctrpi, d-ribose-5-phosphate isomerase, ribosephosphate isomerase b, ribosephosphate isomerase a, ribose-5-phosphate isomerase b, ribose 5-phosphate isomerase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ribose-5-phosphate isomerase
-
Tc00.1047053509199.24
-
type B ribose 5-phosphate isomerase
-
5-Phosphoribose isomerase
-
-
-
-
D-Ribose 5-phosphate isomerase
-
-
-
-
D-ribose-5-phosphate ketol-isomerase
-
-
-
-
D-xylose ketol-isomerase
-
-
-
-
Isomerase, ribose phosphate
-
-
-
-
Phosphopentoisomerase
-
-
-
-
Phosphopentose isomerase
-
-
-
-
Phosphoriboisomerase
-
-
-
-
Ribose phosphate isomerase
-
-
-
-
Ribose-5-P isomerase
-
-
-
-
Ribosephosphate isomerase A
-
-
-
-
Ribosephosphate isomerase B
-
-
-
-
RPI
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
-
intramolecular oxidoreduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-ribose-5-phosphate aldose-ketose-isomerase
Also acts on D-ribose 5-diphosphate and D-ribose 5-triphosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-83-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-ribose 5-diphosphate
D-ribulose 5-diphosphate
show the reaction diagram
-
-
-
r
D-ribulose 5-diphosphate
D-ribose 5-diphosphate
show the reaction diagram
-
-
-
r
D-ribulose 5-phosphate
D-ribose 5-phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R,3S)-3-hydroxy-1-(6-oxo-1,4,5,6-tetrahydropyridazine-3-carbonyl)pyrrolidine-2-carboxylic acid
inhibitor identified by in silico analysis, shows best docking score of -12.735 forming six hydrogen bonds and one salt bridge
4-phospho-D-erythronate
-
4-phospho-D-erythronhydrazide
-
4-phospho-D-erythronohydroxamic acid
competitive
5-phospho-D-ribonohydroxamic acid
-
D-Allose 6-phosphate
weak competitive inhibitor
iodoacetamide
5 mM, complete inhibition
[(2R,3S,4R,5R,6S)-3,4,5,6-tetrahydroxyoxan-2-yl]methyl hydrogen sulfate
inhibitor identified by in silico analysis, second best hit with dock-ing score of -11.927 and is predict to make eight hydrogenbonds, two electrostatic interactions and one possible pi-pi interaction
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2 - 10
D-Ribose 5-diphosphate
1.4 - 2.5
D-ribulose 5-diphosphate
5
D-ribulose 5-phosphate
in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1 - 97
D-Ribose 5-diphosphate
4.7 - 10.7
D-ribulose 5-diphosphate
2 - 8
D-ribulose 5-phosphate
in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
4-phospho-D-erythronohydroxamic acid
pH 8.4, 25°C
15
D-Allose 6-phosphate
in 50 mM Tris-HCl (pH 7.6), 150 mM NaCl and 5 mM MESNA, at 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
4-phospho-D-erythronate
Trypanosoma cruzi
pH 8.4, 25°C
0.7
4-phospho-D-erythronohydroxamic acid
Trypanosoma cruzi
pH 8.4, 25°C
additional information
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression
Manually annotated by BRENDA team
metacyclic and in cell-culture
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q4CQE2_TRYCC
Trypanosoma cruzi (strain CL Brener)
159
0
17362
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17400
2 * 17400, calculated, 2 * 18000, SDS-PAGE
18000
2 * 17400, calculated, 2 * 18000, SDS-PAGE
39600
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 17400, calculated, 2 * 18000, SDS-PAGE
tetramer
x-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
RpiB (wild type or C69A mutant enzyme), sitting drop vapor diffusion method, using 0.5% (v/v) Jeffamine ED-2001, 0.1 M HEPES, 1.1 M Na-malonate (pH 7.0) for the wild type enzyme in complex with phosphate, or 0.8 M Na?K phosphate (pH 8.2) for the mutant enzyme C69A in complex with phosphate, or 20% (w/v) poly(ethyleneglycol) 550 monomethyl ether, 0.1 M NaCl, 0.1 M bicine (pH 9.0) for the wild type enzyme in complex with D-ribose 5-phosphate, or 20% (w/v) poly(ethylene glycol) 6000, 0.2 M ammonium chloride, 0.1 M Tris-HCl (pH 8.0) for wild type enzyme in complex with 4-phospho-D-erythronohydroxamic acid, or 20% (w/v) poly(ethylene glycol) 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane (pH 8.5) for mutant enzyme C69A in complex with allose 6-phosphate
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H102A
kinetics severeyl impaired with substrate ribose 5-phosphate, but not affected with substrate ribulose 5-phosphate
H11A
6fold increase in Km value, 8fold increase in kcat value, decrease in stability to freezing and thawing
H138A
little variations in kinetics compared to wild-type
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Superdex 75 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli ER2566 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Stern, A.L.; Burgos, E.; Salmon, L.; Cazzulo, J.J.
Ribose 5-phosphate isomerase type B from Trypanosoma cruzi: kinetic properties and site-directed mutagenesis reveal information about the reaction mechanism
Biochem. J.
401
279-285
2007
Trypanosoma cruzi (Q4CQE2), Trypanosoma cruzi, Trypanosoma cruzi CL Brener (Q4CQE2)
Manually annotated by BRENDA team
Stern, A.L.; Naworyta, A.; Cazzulo, J.J.; Mowbray, S.L.
Structures of type B ribose 5-phosphate isomerase from Trypanosoma cruzi shed light on the determinants of sugar specificity in the structural family
FEBS J.
278
793-808
2011
Trypanosoma cruzi (Q4CQE2), Trypanosoma cruzi, Trypanosoma cruzi CL Brener (Q4CQE2)
Manually annotated by BRENDA team
de V. C. Sinatti, V.; R. Baptista, L.; Alves-Ferreira, M.; Dardenne, L.; Hermxadnio Martins da Silva, J.; Guimarães, A.
In silico identification of inhibitors of ribose 5-phosphate isomerase from Trypanosoma cruzi using ligand and structure based approaches
J. Mol. Graph. Model.
77
168-180
2017
Trypanosoma cruzi (Q4CQE2), Trypanosoma cruzi, Trypanosoma cruzi CL Brener (Q4CQE2)
Manually annotated by BRENDA team