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Information on EC 5.3.1.4 - L-arabinose isomerase and Organism(s) Yersinia pestis and UniProt Accession P58540

for references in articles please use BRENDA:EC5.3.1.4
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IUBMB Comments
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) . The enzyme binds beta-L-arabinopyranose and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism . The enzyme can also convert alpha-D-galactose to D-tagatose with lower efficiency .
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This record set is specific for:
Yersinia pestis
UNIPROT: P58540
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Word Map
The taxonomic range for the selected organisms is: Yersinia pestis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
l-arabinose isomerase, arabinose isomerase, l-ai us100, l-ai nc8, d-galactose isomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L-arabinose isomerase
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Isomerase, L-arabinose
-
-
-
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L-arabinose ketol-isomerase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
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isomerization
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-
-
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intramolecular oxidoreduction
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-L-arabinopyranose aldose-ketose-isomerase
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) [2]. The enzyme binds beta-L-arabinopyranose [4] and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism [6]. The enzyme can also convert alpha-D-galactose to D-tagatose with lower efficiency [5].
CAS REGISTRY NUMBER
COMMENTARY hide
9023-80-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-galactose
D-tagatose
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-galactose
D-tagatose
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
serves as catalyst
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ARAA_YERPE
500
0
55599
Swiss-Prot
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
there has been industrial interest in the end product D-tagatose as a low-calorie sugar-substituting sweetener
additional information
additionally, tagatose can potentially be used as a prescription drug additive to mask unpleasant tastes, and as a sweetener in toothpaste, mouthwash, and cosmetics such as flavoured lipstick
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, P.
Current studies on biological tagatose production using L-arabinose isomerase: a review and future perspective
Appl. Microbiol. Biotechnol.
65
243-249
2004
Bacillus subtilis (P94523), Bacteroides thetaiotaomicron (Q8AAW1), Bifidobacterium longum (Q8G7J3), Clostridium acetobutylicum (Q97JE0), Clostridium acetobutylicum (Q97JE4), Escherichia coli (P08202), Escherichia coli (P58538), Escherichia coli (Q8FL89), Geobacillus stearothermophilus, Halalkalibacterium halodurans (Q9KBQ2), Klebsiella aerogenes, Lactiplantibacillus plantarum (Q88S84), Lactobacillus gayonii, Oceanobacillus iheyensis (Q8EMP4), Salmonella enterica subsp. enterica serovar Typhi (P58539), Salmonella enterica subsp. enterica serovar Typhimurium (P06189), Shigella flexneri (Q7UDT4), Thermoanaerobacter mathranii, Thermotoga maritima (Q9WYB3), Thermotoga neapolitana, Thermus sp., Vibrio parahaemolyticus, Yersinia pestis (P58540)
Manually annotated by BRENDA team