Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 5.3.1.23 - S-methyl-5-thioribose-1-phosphate isomerase and Organism(s) Homo sapiens and UniProt Accession Q9BV20

for references in articles please use BRENDA:EC5.3.1.23
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q9BV20 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
mtr-1-p, ph0702, ypr118w, methylthioribose-1-phosphate isomerase, 5'-methylthioribose-1-phosphate isomerase, 5-methylthioribose-1-phosphate isomerase, 5-methylthioribose 1-phosphate isomerase, bs-m1pi, 1-pmtr isomerase, mtr-1-p isomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methylthioribose-1-phosphate isomerase
-
MTR-1-P isomerase
-
1-PMTR isomerase
-
-
-
-
5'-methylthioribose-1-phosphate isomerase
-
-
5-methylthio-5-deoxy-D-ribose-1-phosphate ketol-isomerase
-
-
-
-
5-methylthioribose-1-phosphate isomerase
-
-
-
-
Isomerase, methylthioribose 1-phosphate
-
-
-
-
methylthioribose-1-phosphate isomerase
-
-
MRDI
-
mediator of Rho-dependent invasion
mtnA
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
intramolecular oxidoreduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5-thio-alpha-D-ribose-1-phosphate aldose-ketose-isomerase
-
CAS REGISTRY NUMBER
COMMENTARY hide
91608-95-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
show the reaction diagram
-
-
-
?
5-Methylthio-5-deoxy-D-ribose 1-phosphate
5-Methylthio-5-deoxy-D-ribulose 1-phosphate
show the reaction diagram
-
-
-
?
5-Methylthio-5-deoxy-D-ribose 1-phosphate
?
show the reaction diagram
-
enzyme of the pathway of the conversion of methylthioribose-1-phosphate to methionine
-
-
?
S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
show the reaction diagram
-
-
-
?
5-Methylthio-5-deoxy-D-ribose 1-phosphate
?
show the reaction diagram
-
enzyme of the pathway of the conversion of methylthioribose-1-phosphate to methionine
-
-
?
S-methyl-5-thio-alpha-D-ribose 1-phosphate
S-methyl-5-thio-D-ribulose 1-phosphate
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
RNAi
-
stable knockdown of MRDI is carried out in A-375 cells
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
RhoA
-
MRDI is induced by RhoA and mediates RhoA-dependent cell invasion
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
clone A has 5-methylthioribose-1-phosphate isomerase activity, clone D lacks this enzyme
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
MRDI is induced in metastatic cells by constitutive RhoA activation and promotes cell invasion
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MTNA_HUMAN
369
0
39150
Swiss-Prot
other Location (Reliability: 4)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.7 M sodium citrate and 100 mM imidazole, pH 8.0
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C168S
-
inactive mutant
D248A
-
inactive mutant
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by nickel-nitrilotriacetic acid affinity chromatography, the His-tag is removed by treatment with tobacco etch virus protease
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into pCMV plasmid for expression in mammalian cells and into pMS356 plasmid for esxpression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
RhoA induces expression of MRDI
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ghoda, L.Y.; Savarese, T.M.; Dexter, D.L.; Parks, R.E.; Trackman, P.C.; Abeles, R.H.
Characterization of a defect in the pathway for converting 5'-deoxy-5'-methylthioadenosine to methionine in a subline of a cultured heterogeneous human colon carcinoma
J. Biol. Chem.
259
6715-6719
1984
Homo sapiens
Manually annotated by BRENDA team
Kabuyama, Y.; Litman, E.S.; Templeton, P.D.; Metzner, S.I.; Witze, E.S.; Argast, G.M.; Langer, S.J.; Polvinen, K.; Shellman, Y.; Chan, D.; Shabb, J.B.; Fitzpatrick, J.E.; Resing, K.A.; Sousa, M.C.; Ahn, N.G.
A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme
Mol. Cell. Proteomics
8
2308-2320
2009
Homo sapiens
Manually annotated by BRENDA team
Templeton, P.D.; Litman, E.S.; Metzner, S.I.; Ahn, N.G.; Sousa, M.C.
Structure of mediator of RhoA-dependent invasion (MRDI) explains its dual function as a metabolic enzyme and a mediator of cell invasion
Biochemistry
52
5675-5684
2013
Bacillus subtilis, Homo sapiens (Q9BV20), Homo sapiens
Manually annotated by BRENDA team
Mary, C.; Duek, P.; Salleron, L.; Tienz, P.; Bumann, D.; Bairoch, A.; Lane, L.
Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway
PLoS ONE
7
e52877
2012
Homo sapiens
Manually annotated by BRENDA team