Information on EC 5.3.1.21 - corticosteroid side-chain-isomerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.3.1.21
-
RECOMMENDED NAME
GeneOntology No.
corticosteroid side-chain-isomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
11-Deoxycorticosterone = 20-hydroxy-3-oxopregn-4-en-21-al
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
11-deoxycorticosterone aldose-ketose-isomerase
An epimerization at C-20 and C-21 is probably catalysed by the same enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
75139-73-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cow
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
11-Deoxycorticosterone
20-Hydroxy-3-oxopregn-4-en-21-al
show the reaction diagram
17alpha-Hydroxy-11-deoxycorticosterone
17alpha,20-Dihydroxy-3-oxopregn-4-en-21-al
show the reaction diagram
-
-
-
-
-
20beta-Hydroxy-3-oxopregn-4-en-21-al
20alpha-Hydroxy-3-oxopregn-4-en-21-al
show the reaction diagram
-
-
-
-
Corticosterone
11beta,20-Dihydroxy-3-oxopregn-4-en-21-al
show the reaction diagram
-
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
stimulates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
8-hydroxychinoline
-
-
Dithizone
-
-
EDTA
-
reactivation by Co2+, Fe2+, Mo5+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,2'-dipyridyl
-
increases activity
cyanide
-
increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
11-deoxycorticosterone
-
-
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.6
-
acetate/phosphate buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23500
-
2 * 23500, SDS-PAGE
44000
-
gel filtration
389000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 23500, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no glycoprotein
-
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
30 min, stable
58
-
10 min, about 20% loss of activity
65
-
10 min, about 45% loss of activity
100
-
60 s, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-4°C, 0.01 M sodium phosphate, pH 7.0, less than 10% loss of activity after 6 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-