Information on EC 5.3.1.20 - ribose isomerase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
5.3.1.20
-
RECOMMENDED NAME
GeneOntology No.
ribose isomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-Ribose = D-ribulose
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular oxidoreduction
-
-
-
-
isomerization
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
D-ribose aldose-ketose-isomerase
Also acts on L-lyxose and L-rhamnose.
CAS REGISTRY NUMBER
COMMENTARY hide
57534-76-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Allose
?
show the reaction diagram
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-
-
-
-
D-Lyxose
?
show the reaction diagram
-
-
-
-
-
D-Ribose
D-Ribulose
show the reaction diagram
L-Rhamnose
L-Rhamnulose
show the reaction diagram
-
-
-
-
-
additional information
?
-
-
enzyme of the pentose metabolism
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
enzyme of the pentose metabolism
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
divalent cation required, Km: 0.0004 mM
Mg2+
-
divalent cation required, Km: 0.018 mM
Mn2+
-
divalent cation required for activity, Mn2+ is the best activator, Km: 0.0001 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-arabitol
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.3
L-Lyxose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.5
-
pH 6.5: about 45% of maximal activity, pH 9.5: about 55% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
145000 - 174000
-
sedimentation equilibrium analysis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 42000-44000, SDS-PAGE in presence of Mn2+
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85
-
60 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
sensitive to freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
5°C, crystalline enzyme in 44% ammonium sulphate, stable for at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE