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Information on EC 5.2.1.4 - maleylpyruvate isomerase

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UNIPROT: Q8NLC1 not found.
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
msh gene, maleylpyruvate isomerase, mycothiol-dependent maleylpyruvate isomerase, mdmpi, thiol-dependent maleylpyruvate isomerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-maleylpyruvate cis-trans-isomerase
-
maleylpyruvate isomerase
-
MDMPI
mycothiol dependent maleylpyruvate isomerase
Isomerase, maleylpyruvate
-
-
-
-
MPI
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cis-trans-isomerization
SYSTEMATIC NAME
IUBMB Comments
3-maleylpyruvate cis-trans-isomerase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9023-77-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-maleylpyruvate +
3-fumarylpyruvate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
complete inhibitory at 10 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
weak activator at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
148.4
3-maleylpyruvate
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8NLC1_CORGL
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
241
0
26449
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
SDS-PAGE, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 x 33000, SDS-Page
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the wild type and one mutated enzyme (H52A), enzyme contains two domains
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C61A
site directed mutagenesis
D151A
site directed mutagenesis, completely inactive
E144A
site directed mutagenesis, completely inactive
E85A
site directed mutagenesis
H148A
site directed mutagenesis, completely inactive
H48A
site directed mutagenesis
H52A
site directed mutagenesis, completely inactive
N56A
site directed mutagenesis, completely inactive
R141A
site directed mutagenesis, completely inactive
R222A
site directed mutagenesis, completely inactive
R82A
site directed mutagenesis, partially inactive
S78A
site directed mutagenesis, almost completely inactive
W44A
site directed mutagenesis, completely inactive
Y76A
site directed mutagenesis, completely inactive
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
at -80C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the recombinant enzyme
of the recombinant wild type and mutant enzymes
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
mycothiol as target for clinical treatment
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Feng, J.; Che, Y.; Milse, J.; Yin, Y.J.; Liu, L.; Rueckert, C.; Shen, X.H.; Qi, S.W.; Kalinowski, J.; Liu, S.J.
The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in Corynebacterium glutamicum
J. Biol. Chem.
281
10778-10785
2006
Corynebacterium glutamicum (Q8NLC1), Corynebacterium glutamicum
Manually annotated by BRENDA team
Wang, R.; Yin, Y.J.; Wang, F.; Li, M.; Feng, J.; Zhang, H.M.; Zhang, J.P.; Liu, S.J.; Chang, W.R.
Crystal structures and site-directed mutagenesis of a mycothiol-dependent enzyme reveal a novel folding and molecular basis for mycothiol-mediated maleylpyruvate isomerization
J. Biol. Chem.
282
16288-16294
2007
Corynebacterium glutamicum (Q8NLC1), Corynebacterium glutamicum
Manually annotated by BRENDA team